{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T22:00:03Z","timestamp":1790978403431,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,3,28]],"date-time":"2002-03-28T00:00:00Z","timestamp":1017273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2002,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mantle cell lymphoma (MCL) is a B\u2010lymphocytic malignancy frequently associated with the presence of the t(11;14) chromosomal translocation. By using a polymerase chain reaction (PCR) strategy to detect breakpoints within the major translocation cluster (MTC), an unexpectedly large product (about 1.1\u2009kb by using first\u2010round bcl\u20101\/JH primers) has been identified in one out of 16 patients harbouring the t(11;14) translocation. Sequence analysis of the atypical PCR product, re\u2010amplified and cloned with second\u2010round primers, revealed a 459\u2009bp portion corresponding exactly to the 3\u2032\u2010end segment of the MTC, followed by a sequence of 433\u2009bp that lacked homology with any previously known sequence. PCR experiments using DNA from healthy donors identified that fragment as an extension of MTC fused, through a N\u2010region of seven nucleotides, to the JH4 region of IgH gene. A computer\u2010based search of the novel MTC portion aimed at detecting potential recombination motifs revealed the presence of several 4\u2010bp sequences (5\u2032\u2010CCAG\u20103\u2032 or its complement 5\u2032\u2010CTGG\u20103\u2032), one of them within seven nucleotides from the putative breakpoint, known to play a role in non\u2010homologous recombination events at the Ig loci. The recognition of this novel breakpoint may have important implications for the diagnosis and detection of minimal residual disease in t(11;14)\u2010positive lymphomas. 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