{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:39:07Z","timestamp":1761093547323,"version":"build-2065373602"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,3,19]],"date-time":"2002-03-19T00:00:00Z","timestamp":1016496000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2002,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nerve growth factor (NGF) regulates the survival and development of specific populations of neurones and is involved in wound healing. A further area of study relating to the role of neurotrophins in the mature animal has concerned the possibility that NGF may be a pivotal mediator of inflammation and pain. It has previously been shown that injection of intradermal NGF can result in a neutrophil\u2010dependent hyperalgesia in the rat. The purpose of the present study was to examine the pathological consequence of NGF injected intradermally into mature rat skin and to examine further the role of neutrophils. Standard histopathology techniques (H &amp; E) were employed to determine inflammatory cell counts. Circulating neutrophils were depleted using an anti\u2010rat neutrophil antiserum and results were compared to treatment with vehicle controls. Saline\u2010pretreated rats exhibited normal circulating neutrophil numbers and the dorsal skin showed a significant increase of neutrophil and macrophages at 3 and 5\u2009h and lymphocytes at 5\u2009h after NGF treatment. By comparison, skin sites from neutrophil\u2010depleted rats did not demonstrate a significant increase in neutrophil and macrophage accumulation after NGF administration. All NGF\u2010treated sites, independent of pretreatment, demonstrated abnormal muscle fibre morphology and proliferation of the muscle sarcolemmal nuclei after NGF injection, indicative of tissue injury. In addition, oedema and some fibroplasia were also noted. Furthermore, fibrin production was increased at 3 and 5\u2009h after NGF administration. It is suggested that NGF has a damaging effect on rat muscle which is independent of accumulating neutrophil and other inflammatory cells. In conclusion, the findings indicate a link between NGF\u2010induced neutrophil and macrophage accumulation, as the increase in dermal macrophages was not observed in neutrophil\u2010depleted rats. The results also suggest that NGF can have a profound effect on rat muscle and that this effect may be related to muscle regeneration. Copyright \u00a9 2002 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.1111","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T08:20:06Z","timestamp":1033892406000},"page":"245-255","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Cellular pathology changes in rat skin following intradermal injection of nerve growth factor: neutrophil\u2010dependent and \u2010independent events"],"prefix":"10.1002","volume":"197","author":[{"given":"Paul A.","family":"Foster","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharon","family":"Wicks","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martyn","family":"Foster","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan D.","family":"Brain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,3,19]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1968.48.3.534"},{"key":"e_1_2_6_3_2","first-page":"82","article-title":"Neurotrophic factors and neuronal death","volume":"126","author":"Thoenen H","year":"1987","journal-title":"Ciba Found Symp"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1996.0039"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0895-774"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-12-07929.1995"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(87)90250-1"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/326353a0"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-03-01422.1994"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12388914"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)54695-0"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199411000-00019"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.20.10955"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780350315"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.134.1.564"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(93)90092-Z"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(98)00114-6"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.23.7215"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1080\/000155598433368"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4800(85)90048-6"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.3.297"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.7.4379"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.159.1.383"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1979.tb03001.x"},{"key":"e_1_2_6_25_2","first-page":"75","article-title":"Untersuchungen uber rheumatische Myokarditis","volume":"85","author":"Giepel P","year":"1906","journal-title":"Deutsch Arch Klin Med"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711380405"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092400415"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.05-11-03025.1985"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006992126707"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V88.12.4630.bloodjournal88124630"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1996.0142"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(99)00306-1"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-8703(99)70323-6"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410360221"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.48.2.501"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1177\/096368979800700609"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-200001000-00028"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.82B1.0820131"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.1111","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.1111","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T12:00:06Z","timestamp":1761048006000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.1111"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,3,19]]},"references-count":37,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2002,6]]}},"alternative-id":["10.1002\/path.1111"],"URL":"https:\/\/doi.org\/10.1002\/path.1111","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"type":"print","value":"0022-3417"},{"type":"electronic","value":"1096-9896"}],"subject":[],"published":{"date-parts":[[2002,3,19]]},"assertion":[{"value":"2001-08-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-01-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-03-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}