{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T22:21:03Z","timestamp":1782253263220,"version":"3.54.5"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2002,5,8]],"date-time":"2002-05-08T00:00:00Z","timestamp":1020816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2002,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Epstein\u2013Barr virus (EBV) is known to be associated with a variety of tumours, including Burkitt's lymphoma, nasopharyngeal carcinoma, and some carcinomas of other organs with similar lymphoepithelioma\u2010like features. The association between EBV and thymic epithelial tumours is inconclusive, as reports in this regard are not entirely consistent and the methods employed are of different sensitivity and specificity. This study examined 78 thymomas and 21 thymic carcinomas in Taiwanese patients, to detect the viral genome at both DNA and RNA levels. The tissue blocks were first screened by nested polymerase chain reaction (PCR) targeting on the first tandem internal repeats. The positive cases were further submitted for viral localization by <jats:italic>in situ<\/jats:italic> PCR <jats:italic>insitu<\/jats:italic> hybridization (ISH) and Epstein\u2013Barr\u2010encoded RNA\u20101 (EBER\u20101) ISH. None of the thymomas showed a detectable EBV genome. Eight thymic carcinomas were positive for EBV by nested PCR, of which six displayed nuclear signals within the tumour cells by <jats:italic>in situ<\/jats:italic> PCR ISH and\/or RNA ISH, one displayed signals within the lymphocytes, and one showed no discernible <jats:italic>in situ<\/jats:italic> signals. Most of them exhibited a lymphoepithelioma\u2010like morphology. These results show that nested PCR is a sensitive method for screening the EBV genome in thymic epithelial tumours. <jats:italic>In situ<\/jats:italic> PCR ISH is reliable for localization of the virus, in addition to EBER\u20101 RNA ISH. Thymomas are not related to EBV, even in this endemic area. Thymic carcinomas, especially the lymphoepithelioma\u2010like thymic carcinomas, are more often associated with the virus. Copyright \u00a9 2002 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.1141","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T08:07:53Z","timestamp":1033891673000},"page":"684-688","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Detection of Epstein\u2013Barr virus genome within thymic epithelial tumours in Taiwanese patients by nested PCR, PCR <i>in situ<\/i> hybridization, and RNA <i>in situ<\/i> hybridization"],"prefix":"10.1002","volume":"197","author":[{"given":"Paul Chih\u2010Hsueh","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chin\u2010Chen","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"An\u2010Hang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang\u2010Shun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hung","family":"Chiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,5,8]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200008173430707"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198505163122006"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19880615)61:12<2475::AID-CNCR2820611215>3.0.CO;2-5"},{"key":"e_1_2_6_5_2","first-page":"1429","article-title":"Western thymomas lack Epstein\u2013Barr virus by Southern blotting analysis and by polymerase chain reaction","volume":"136","author":"Inghirami G","year":"1990","journal-title":"Am J Pathol"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02899425"},{"key":"e_1_2_6_7_2","first-page":"363","article-title":"In situ localization of Epstein\u2013Barr virus in thymic carcinoma","volume":"5","author":"Mann RB","year":"1992","journal-title":"Mod Pathol"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0463.2000.d01-74.x"},{"key":"e_1_2_6_9_2","first-page":"385","article-title":"Epstein\u2013Barr virus genome in thymoma and thymic lymphoid hyperplasia","volume":"131","author":"McGuire LJ","year":"1988","journal-title":"Am J Pathol"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.1992.tb00075.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(93)90031-B"},{"key":"e_1_2_6_12_2","volume-title":"PCR in situ Hybridization. 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