{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T03:10:03Z","timestamp":1761102603525,"version":"build-2065373602"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2002,8,2]],"date-time":"2002-08-02T00:00:00Z","timestamp":1028246400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2002,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The cDNA clone pCMa1 (0.45\u2009kb) is one of the 12 novel cDNAs, previously identified when comparing RNA expression profiles of melanocytes, naevus cells, and non\u2010metastatic melanoma cells. This clone did not reveal a unique long open reading frame. The pCMa1 gene localized to the distal, telomere proximal region on the short arm of chromosome 11.p15.1\u20132. Northern blot analyses with single\u2010stranded cRNA probes revealed the presence of various complementary pCMa1 transcripts of different lengths, which are not enriched in the poly(A)<jats:sup>+<\/jats:sup> RNA fraction. The arbitrarily defined <jats:italic>plus<\/jats:italic> strand (used as a probe) mainly hybridized to 0.45\u2009kb and 4.0\u2009kb <jats:italic>minus<\/jats:italic> transcripts in total RNA samples, and the <jats:italic>minus<\/jats:italic> strand (used as a probe) hybridized to a major <jats:italic>plus<\/jats:italic> transcript of 4.0\u2009kb. By RNA <jats:italic>in situ<\/jats:italic> hybridization, the highest levels of the <jats:italic>plus<\/jats:italic> transcripts were observed in melanocytic naevi (12\/12), particularly in congenital naevi, whereas normal skin melanocytes (12\/12) were negative. pCMa1 plus transcripts were detected in naevus cell nests (100%) near the dermo\u2010epidermal junction. Expression, however, diminished to some extent in the deeper parts of the melanocytic naevi. Although most of the cutaneous primary melanoma lesions (11\/15) showed detectable, but variable levels of <jats:italic>plus<\/jats:italic> transcripts of pCMa1 in the papillary to reticular dermis, not more than 10% of the melanoma cells were positive. The majority of melanoma metastases (6\/7) were negative, while the positive lesion originated from a patient with a positive primary melanoma. Furthermore, <jats:italic>plus<\/jats:italic> transcripts were present in the nuclei of non\u2010metastatic melanoma cells in culture, whereas metastatic cells showed elevated expression both in the nucleus and in the cytoplasm. Briefly, the data show transient up\u2010regulation of pCMa1 in neoplastic progression of melanocytic cells, with peak levels occurring during naevus stages, and suggest that pCMa1 is a molecular marker in melanocytes for the early changes from the proliferating phenotype to malignant transformation. Copyright \u00a9 2002 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.1152","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T08:07:53Z","timestamp":1033891673000},"page":"668-676","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multiple complementary transcripts of pCMa1, a novel gene located at chromosome 11p15.1\u20132, and melanocytic cell transformation"],"prefix":"10.1002","volume":"197","author":[{"given":"Clifton B.","family":"Meije","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pranab K.","family":"Das","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miranda M. E.","family":"Jans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi","family":"Hau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allard C.","family":"van der Wal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marielle","family":"Alders","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theodorus B. M.","family":"Hakvoort","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich H.","family":"Weidle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wouter H.","family":"Lamers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guido W. M.","family":"Swart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,8,2]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(96)90041-9"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800515"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(84)80310-X"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)60648-X"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19970611)71:6<1035::AID-IJC20>3.0.CO;2-B"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.17.9160"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19960208)65:4<460::AID-IJC12>3.0.CO;2-E"},{"key":"e_1_2_6_9_2","first-page":"5266","article-title":"Identification of genes differentially expressed in association with metastatic potential of K\u20101735 murine melanoma by messenger RNA differential display","volume":"56","author":"Hashimoto Y","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_6_10_2","first-page":"3855","article-title":"Identification by differential display of annexin\u2010VI, a gene differentially expressed during melanoma progression","volume":"56","author":"Francia G","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_6_11_2","first-page":"6237","article-title":"Identification of melanoma inhibitory activity and other differentially expressed messenger RNAs in human melanoma cell lines with different metastatic capacity by messenger RNA differential display","volume":"55","author":"van Groningen JJ","year":"1995","journal-title":"Cancer Res"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2168.1997.02657.x"},{"key":"e_1_2_6_13_2","first-page":"875","article-title":"Identification of genes differentially expressed in B16 murine melanoma sublines with different metastatic potentials","volume":"56","author":"Ishiguro T","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1017"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(200004)190:5<572::AID-PATH570>3.0.CO;2-9"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01753729"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910480116"},{"volume-title":"Molecular Cloning: a Laboratory Manual","year":"1989","author":"Sambrook J","key":"e_1_2_6_18_2"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.26.12456"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00159117"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1159\/000472337"},{"key":"e_1_2_6_22_2","first-page":"5091","article-title":"Antigenic profile of tumor progression stages in human melanocytic nevi and melanomas","volume":"49","author":"Elder DE","year":"1989","journal-title":"Cancer Res"},{"key":"e_1_2_6_23_2","first-page":"1743s","article-title":"Imprinting of a genomic domain of 11p15 and loss of imprinting in cancer: an introduction","volume":"59","author":"Feinberg AP","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-200010000-00008"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2264(200005)28:1<1::AID-GCC1>3.0.CO;2-#"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.090087497"},{"key":"e_1_2_6_27_2","first-page":"130","article-title":"The role of Wilms' tumor genes","volume":"46","author":"Hirose M","year":"1999","journal-title":"J Med Invest"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/7.5.895"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.2741\/A335"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1998.5221"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)00534-0"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.1152","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.1152","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T13:48:43Z","timestamp":1761054523000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.1152"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,8]]},"references-count":30,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2002,8]]}},"alternative-id":["10.1002\/path.1152"],"URL":"https:\/\/doi.org\/10.1002\/path.1152","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"type":"print","value":"0022-3417"},{"type":"electronic","value":"1096-9896"}],"subject":[],"published":{"date-parts":[[2002,8]]},"assertion":[{"value":"2001-08-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-03-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-08-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}