{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:51:13Z","timestamp":1786978273022,"version":"build-2736575974"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T00:00:00Z","timestamp":1027641600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    By prospectively studying immunoglobulin heavy chain gene (\n                    <jats:italic>IgH)<\/jats:italic>\n                    and T cell receptor gamma (\n                    <jats:italic>TCR<\/jats:italic>\n                    \u03b3) gene rearrangements in 398 lymphoma cases, a dual genotype was observed in 13% of B cell and 11% of T cell lymphomas. According to histological subtype, the highest incidence was observed for mantle cell lymphomas (32%) and lymphoplasmacytic lymphoma (21%) among B cell lymphomas, and for angioimmunoblastic lymphoma (AILT) (46%) and S\u00e9zary syndrome (SS) (50%) among T cell lymphomas. To determine whether the dual genotype corresponds to the presence of two distinct monoclonal populations or to the presence of both rearrangements within the same lymphoma cells, single\u2010cell microdissection was used after immunohistochemistry and a single\u2010cell combined\n                    <jats:italic>IgH<\/jats:italic>\n                    and\n                    <jats:italic>TCR<\/jats:italic>\n                    \u03b3 gene analysis was designed after a whole\u2010genome amplification step. This protocol was applied to the study of two nodal B cell lymphomas (one diffuse large B cell lymphoma and one mantle cell lymphoma) and two cutaneous T cell lymphomas (one AILT and one SS). Two cases (SS and mantle cell lymphoma) were true bigenotypic lymphomas, as both\n                    <jats:italic>IgH<\/jats:italic>\n                    and\n                    <jats:italic>TCR<\/jats:italic>\n                    \u03b3 monoclonal rearrangements were detected in the same cells. Conversely, in the diffuse large B cell lymphoma and AILT cases, large CD22+ single cells exhibited only the monoclonal\n                    <jats:italic>IgH<\/jats:italic>\n                    rearrangement but not the\n                    <jats:italic>TCR<\/jats:italic>\n                    \u03b3 gene that was detected in CD3+ single cells. Such an approach allows the identification of true bigenotypic lymphoma among dual genotypic lymphoma. Specific genetic alterations may be further amplified from microdissected cryopreserved material, such as the t(11;14) breakpoint detected in bigenotypic B cells of the mantle cell lymphoma case. Copyright \u00a9 2002 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/path.1192","type":"journal-article","created":{"date-parts":[[2002,10,8]],"date-time":"2002-10-08T23:09:42Z","timestamp":1034118582000},"page":"171-180","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Combined analysis of T cell receptor \u03b3 and immunoglobulin heavy chain gene rearrangements at the single\u2010cell level in lymphomas with dual genotype"],"prefix":"10.1002","volume":"198","author":[{"given":"B.","family":"Vergier","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Dubus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Kutschmar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Parrens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Ferrer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"de Mascarel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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