{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:43:02Z","timestamp":1790826182396,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,7,24]],"date-time":"2002-07-24T00:00:00Z","timestamp":1027468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Helsinki University Central Hospital Research Foundation (EVO)"},{"name":"Turku University Central Hospital"},{"DOI":"10.13039\/501100003125","name":"Finnish Cultural Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003125","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Maud Kuistila Foundation"},{"DOI":"10.13039\/501100002341","name":"Academy of Finland","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Sigrid Jus\u00e9lius Foundation"},{"DOI":"10.13039\/100010135","name":"Finska L\u00e4kares\u00e4llskapet","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100010135","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Cancer Research Foundation of Finland"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human metalloelastase (MMP\u201012) has been implicated in elastin degradation and macrophage migration in many pathological conditions. It also generates angiostatin, thus having a potential to prevent tumour angiogenesis. It has previously been shown that transformed epithelial cells express MMP\u201012 in skin cancer. The aim of this study was further to elucidate the role of metalloelastase in squamous cell cancer (SCC) progression. By <jats:italic>in situ<\/jats:italic> hybridization, expression of MMP\u201012 mRNA was detected in 28\/33 vulvar SCC samples in CD\u201068\u2010positive macrophages, while 10 samples had positive cancer cells. By immunohistochemistry, MMP\u201012 protein was seen in the same area as the mRNA. MMP\u201012 mRNA expression in tumour cells correlated with more aggressive histology (<jats:italic>p<\/jats:italic> = 0.0099). In contrast, macrophage\u2010derived MMP\u201012 mRNA was more abundant in well\u2010differentiated grade I than grade III tumours (<jats:italic>p<\/jats:italic> = 0.01). However, the level of MMP\u201012 mRNA, regardless of its origin, did not correlate with metastasis or patient survival. No significant correlation was found between macrophage\u2010derived MMP\u201012 mRNA and a low amount of blood vessels, as quantitated after von Willebrand staining. In agreement with vulvar SCCs <jats:italic>in vivo<\/jats:italic>, MMP\u201012 was expressed in cultured SCC cells by northern and western blot analysis. In HaCaTs and epithelial MCF\u201010f cells, MMP\u201012 mRNA was induced by transforming growth factor\u2010\u03b21 (TGF\u2010\u03b21) and tumour necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) as measured by quantitative RT\u2010PCR (TaqMan). Two MMPs capable of generating angiostatin <jats:italic>in vivo<\/jats:italic>, matrilysin (MMP\u20107) and gelatinase B (MMP\u20109), were also examined in these tumours. MMP\u20107 mRNA was mainly expressed by epithelial tumour cells, particularly in less differentiated tumours. MMP\u20109 was usually expressed by neutrophils and macrophages; epithelial protein was predominantly found in grade II\/III tumours. These results suggest a dual role for MMP\u201012 in tumour progression. Copyright \u00a9 2002 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.1198","type":"journal-article","created":{"date-parts":[[2002,10,8]],"date-time":"2002-10-08T23:09:42Z","timestamp":1034118582000},"page":"258-269","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":83,"title":["Metalloelastase (MMP\u201012) expression by tumour cells in squamous cell carcinoma of the vulva correlates with invasiveness, while that by macrophages predicts better 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