{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:18:17Z","timestamp":1773285497831,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,10,8]],"date-time":"2002-10-08T00:00:00Z","timestamp":1034035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Wound healing is a complex process of which growth and motility are essential features. The aim of this study was to search for keratinocyte\u2010derived secreted factors that may play a role in these mechanisms, and their corresponding receptors. Growth and motility factors were purified from conditioned medium from cultured primary keratinocytes. Receptor and growth factor expression profiles were investigated by immunohistochemical, western blotting, and\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization analysis on cultured keratinocytes and tissue sections derived from chronic wounds. The most potent autocrine growth factor for keratinocytes, which it was possible to purify and sequence from keratinocyte\u2010conditioned medium, is amphiregulin. Its receptor HER\u20101 is up\u2010regulated on the membranes of keratinocytes lining the edge of the wound. From the same keratinocyte\u2010conditioned medium, heregulin\u2010\u03b1 was purified as a potent motility factor for keratinocytes. Its receptor is HER\u20103, which is up\u2010regulated on the membranes of keratinocytes lining the edge of the wound and on keratinocytes that had migrated towards the centre of the wound. HER\u20104 \u2014 another receptor for heregulin\u2010\u03b1 \u2014 is weakly present in occasional cells near the edge of the wound. The co\u2010receptor for HER\u20103 and HER\u20104 is HER\u20102\/neu, which is also present in epidermal cells but not overexpressed. This study shows that heregulin\u2010\u03b1 is a potent motility factor for normal epithelial cells and that it plays a central role in the process of wound healing of stratified epithelia. Heregulin\u2010\u03b1 has already been shown to be the motility factor leading to migration of HER\u20102\/neu\u2010overexpressing breast cancer cells. The role of amphiregulin as a growth factor and of heregulin\u2010\u03b1 as a motility factor for keratinocytes in epidermal and mucosal wound healing parallels their motility and growth induction in carcinogenesis. Copyright \u00a9 2002 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/path.1240","type":"journal-article","created":{"date-parts":[[2002,11,15]],"date-time":"2002-11-15T10:53:59Z","timestamp":1037357639000},"page":"523-533","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["The role of heregulin\u2010\u03b1 as a motility factor and amphiregulin as a growth factor in wound healing"],"prefix":"10.1002","volume":"198","author":[{"given":"Vera RJ","family":"Schelfhout","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth D","family":"Coene","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Delaey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anouk 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