{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T07:11:09Z","timestamp":1789888269445,"version":"4.0.1"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,3,17]],"date-time":"2003-03-17T00:00:00Z","timestamp":1047859200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Caveolae and their proteins, the caveolins, transport macromolecules; compartmentalize signalling molecules; and are involved in various repair processes. There is little information regarding their role in the pathogenesis of significant renal syndromes such as acute renal failure (ARF). In this study, an <jats:italic>in vivo<\/jats:italic> rat model of 30 min bilateral renal ischaemia followed by reperfusion times from 4 h to 1 week was used to map the temporal and spatial association between caveolin\u20101 and tubular epithelial damage (desquamation, apoptosis, necrosis). An <jats:italic>in vitro<\/jats:italic> model of ischaemic ARF was also studied, where cultured renal tubular epithelial cells or arterial endothelial cells were subjected to injury initiators modelled on ischaemia\u2013reperfusion (hypoxia, serum deprivation, free radical damage or hypoxia\u2013hyperoxia). Expression of caveolin proteins was investigated using immunohistochemistry, immunoelectron microscopy, and immunoblots of whole cell, membrane or cytosol protein extracts. <jats:italic>In vivo<\/jats:italic>, healthy kidney had abundant caveolin\u20101 in vascular endothelial cells and also some expression in membrane surfaces of distal tubular epithelium. In the kidneys of ARF animals, punctate cytoplasmic localization of caveolin\u20101 was identified, with high intensity expression in injured proximal tubules that were losing basement membrane adhesion or were apoptotic, 24 h to 4 days after ischaemia\u2013reperfusion. Western immunoblots indicated a marked increase in caveolin\u20101 expression in the cortex where some proximal tubular injury was located. <jats:italic>In vitro<\/jats:italic>, the main treatment\u2010induced change in both cell types was translocation of caveolin\u20101 from the original plasma membrane site into membrane\u2010associated sites in the cytoplasm. Overall, expression levels did not alter for whole cell extracts and the protein remained membrane\u2010bound, as indicated by cell fractionation analyses. Caveolin\u20101 was also found to localize intensely within apoptotic cells. The results are indicative of a role for caveolin\u20101 in ARF\u2010induced renal injury. Whether it functions for cell repair or death remains to be elucidated. Copyright \u00a9 2003 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.1368","type":"journal-article","created":{"date-parts":[[2003,7,2]],"date-time":"2003-07-02T06:02:58Z","timestamp":1057125778000},"page":"396-405","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["<i>In vivo<\/i> and <i>in vitro<\/i> models demonstrate a role for caveolin\u20101 in the pathogenesis of ischaemic acute renal failure"],"prefix":"10.1002","volume":"200","author":[{"given":"Mitra","family":"Mahmoudi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desley","family":"Willgoss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leila","family":"Cuttle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Betty","family":"Pat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clay","family":"Winterford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoltan","family":"Endre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glenda C","family":"Gob\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,3,17]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.126.1.111"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.10.5419"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004180050335"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.46.29337"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(95)01256-7"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/7.5.871"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.111.6.825"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/002215549804600209"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2001.059002471.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199703203361203"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V113454"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.9790"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1210\/mend.13.12.0392"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2000.740676.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.11.7481"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1615845"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.144.6.1285"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M001131200"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)33898-0"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1999.00745.x"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-201x.2000.00636.x"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2000.00154.x"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2002.00316.x"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.46.29427"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)33898-0"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/10067"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-201x.2000.00629.x"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1080\/10408369991239240"},{"key":"e_1_2_6_30_2","first-page":"770","article-title":"Cellular events in experimental unilateral ischemic renal atrophy and in regeneration after contralateral nephrectomy","volume":"63","author":"Gobe GC","year":"1990","journal-title":"Lab Invest"},{"key":"e_1_2_6_31_2","first-page":"831","article-title":"Morphologic, biochemical, and molecular evidence of apoptosis during the reperfusion phase after brief periods of renal ischemia","volume":"140","author":"Schumer M","year":"1992","journal-title":"Am J Pathol"},{"key":"e_1_2_6_32_2","first-page":"505","article-title":"Necrosis and apoptosis in acute renal failure","volume":"18","author":"Lieberthal W","year":"1998","journal-title":"Semin Nephrol"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2000.00925.x"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.3109\/10623329909165310"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(08)61921-4"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.42.27383"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.148.4.727"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.26.16374"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.1368","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.1368","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T11:45:22Z","timestamp":1761047122000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.1368"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,3,17]]},"references-count":37,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2003,7]]}},"alternative-id":["10.1002\/path.1368"],"URL":"https:\/\/doi.org\/10.1002\/path.1368","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"value":"0022-3417","type":"print"},{"value":"1096-9896","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,3,17]]},"assertion":[{"value":"2002-08-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-01-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-03-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}