{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T09:46:53Z","timestamp":1761126413179,"version":"build-2065373602"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,1,19]],"date-time":"2004-01-19T00:00:00Z","timestamp":1074470400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"crossref","award":["NSC 90-2314-B-182-076"],"award-info":[{"award-number":["NSC 90-2314-B-182-076"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Chang Gung Memorial Hospital, Taiwan","award":["CMRP G8005"],"award-info":[{"award-number":["CMRP G8005"]}]}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Advanced liver cirrhosis frequently occurs in infants with biliary atresia despite early surgical correction. The aetiology is unknown, but may involve many cytokines and liver cells including hepatic stellate cells (HSCs). A cytokine expression array and real\u2010time quantitative reverse transcription\u2010polymerase chain reaction (QRT\u2010PCR) were used to study cytokine expression during the progression of liver fibrosis in biliary atresia. A <jats:italic>Delta\u2010like 1 homologue<\/jats:italic> (<jats:italic>DLK1<\/jats:italic>) gene was identified and this gene was up\u2010regulated during the early stage, and down\u2010regulated during the late stage, of biliary atresia, similar to the expression pattern of the <jats:italic>procollagen \u03b11(I) gene<\/jats:italic>. Further characterization with immunohistochemistry, confocal microscopy, and <jats:italic>in situ<\/jats:italic> hybridization revealed that the DLK1 protein was mainly present in the cytoplasm of smooth muscle actin\u2010positive mesenchymal cells that were morphologically and immunohistochemically identical to activated HSCs\/myofibroblasts, whereas <jats:italic>DLK1<\/jats:italic> mRNA was present only in hepatocytes. As DLK1 is a negative regulator of adipocyte differentiation and may control cell fate during differentiation, overexpression of DLK1 protein in HSCs in the early stage of biliary atresia suggests that DLK1 may be implicated in the transformation of HSCs from fat\u2010storing cells to myofibroblasts and in fibrogenesis associated with biliary atresia. Copyright \u00a9 2004 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.1505","type":"journal-article","created":{"date-parts":[[2004,1,21]],"date-time":"2004-01-21T03:00:59Z","timestamp":1074654059000},"page":"172-179","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["The human Delta\u2010like 1 homologue is implicated in the progression of liver fibrosis in biliary atresia"],"prefix":"10.1002","volume":"202","author":[{"given":"Chao\u2010Cheng","family":"Huang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiin\u2010Haur","family":"Chuang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynn LH","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming\u2010Huei","family":"Chou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chia\u2010Lin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ching\u2010Mei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chie\u2010Song","family":"Hsieh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin\u2010Ye","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao\u2010Long","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,1,19]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-1007164"},{"issue":"5","key":"e_1_2_6_3_2","first-page":"S18","article-title":"Transplantation for childhood liver disease: an overview","volume":"4","author":"Balistreri WF","year":"1998","journal-title":"Liver Transpl Surg"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200006270-00018"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(92)90342-V"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1053\/jpsu.2000.19245"},{"key":"e_1_2_6_7_2","first-page":"107","volume-title":"Pathology of the Liver","author":"Ishak KG","year":"2002"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1536-4801.1998.tb01103.x"},{"key":"e_1_2_6_9_2","first-page":"721","volume-title":"The Liver: Biology and Pathobiology","author":"Rojkind M","year":"2001"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90252-L"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.137.7.2923"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.2.977"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.19-1-71"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.2001.5464"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840200104"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(99)00377-0"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840190629"},{"key":"e_1_2_6_18_2","first-page":"119","article-title":"The role of the epidermal growth factor\u2010like protein dlk in cell differentiation","volume":"15","author":"Laborda J","year":"2000","journal-title":"Histol Histopathol"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/1096-911X(20001201)35:6<554::AID-MPO13>3.0.CO;2-R"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4781(95)00007-4"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)53544-4"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205612"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01324802"},{"key":"e_1_2_6_24_2","first-page":"455","volume-title":"The liver: Biology and Pathobiology","author":"Li D","year":"2001"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.1996.0040"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.3469"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3640137"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1994.00083.x"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510260209"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.1999.884_m.x"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840070609"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)38430-3"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1746.1999.01928.x"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(02)03191-6"},{"key":"e_1_2_6_35_2","first-page":"S39","article-title":"Transdifferentiation of hepatic stellate cells (Ito cells) to myofibroblasts: a key event in hepatic fibrogenesis","volume":"54","author":"Gressner AM","year":"1996","journal-title":"Kidney Int Suppl"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-1007105"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.1505","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.1505","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.1505","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T09:49:02Z","timestamp":1761040142000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.1505"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1,19]]},"references-count":35,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,2]]}},"alternative-id":["10.1002\/path.1505"],"URL":"https:\/\/doi.org\/10.1002\/path.1505","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"type":"print","value":"0022-3417"},{"type":"electronic","value":"1096-9896"}],"subject":[],"published":{"date-parts":[[2004,1,19]]},"assertion":[{"value":"2003-07-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-10-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-01-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}