{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T09:05:33Z","timestamp":1791104733500,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,5,21]],"date-time":"2004-05-21T00:00:00Z","timestamp":1085097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2004,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Array\u2010based comparative genomic hybridization (aCGH) allows the identification of DNA sequence copy number changes at high resolution by co\u2010hybridizing differentially labelled test and control DNAs to a micro\u2010array of genomic clones. The present study has analysed a series of 23 formalin\u2010fixed, paraffin wax\u2010embedded tissue samples of Barrett's adenocarcinoma (BCA, <jats:italic>n<\/jats:italic> = 18) and non\u2010neoplastic squamous oesophageal (<jats:italic>n<\/jats:italic> = 2) and gastric cardia mucosa (<jats:italic>n<\/jats:italic> = 3) by aCGH. The micro\u2010arrays used contained 287 genomic targets covering oncogenes, tumour suppressor genes, and DNA sequences localized within chromosomal regions previously reported to be altered in BCA. DNA sequence copy number changes for a panel of approximately 50 genes were identified, most of which have not been previously described in BCA. DNA sequence copy number gains (mean 41 \u00b1 25\/BCA) were more frequent than DNA sequence copy number losses (mean 20 \u00b1 15\/BCA). The highest frequencies for DNA sequence copy number gains were detected for <jats:italic>SNRPN<\/jats:italic> (61%); <jats:italic>GNLY<\/jats:italic> (44%); <jats:italic>NME1<\/jats:italic> (44%); <jats:italic>DDX15, ABCB1<\/jats:italic> (<jats:italic>MDR<\/jats:italic>), <jats:italic>ATM, LAMA3, MYBL2, ZNF217<\/jats:italic>, and <jats:italic>TNFRSF6B<\/jats:italic> (39% each); and <jats:italic>MSH2, TERC, SERPINE1, AFM137XA11, IGF1R<\/jats:italic>, and <jats:italic>PTPN1<\/jats:italic> (33% each). DNA sequence copy number losses were identified for <jats:italic>PDGFB<\/jats:italic> (44%); <jats:italic>D17S125<\/jats:italic> (39%); <jats:italic>AKT3<\/jats:italic> (28%); and <jats:italic>RASSFI, FHIT, CDKN2A<\/jats:italic> (<jats:italic>p16<\/jats:italic>), and <jats:italic>SAS<\/jats:italic> (<jats:italic>CDK4<\/jats:italic>) (28% each). In all non\u2010neoplastic tissue samples of squamous oesophageal and gastric cardia mucosa, the measured mean ratios were 1.00 (squamous oesophageal mucosa) or 1.01 (gastric mucosa), indicating that no DNA sequence copy number chances were present. For validation, the DNA sequence copy number changes of selected clones (<jats:italic>SNRPN, CMYC, HER2, ZNF217<\/jats:italic>) detected by aCGH were confirmed by fluorescence <jats:italic>in situ<\/jats:italic> hybridization (FISH). These data show the sensitivity of aCGH for the identification of DNA sequence copy number changes at high resolution in BCA. The newly identified genes may include so far unknown biomarkers in BCA and are therefore a starting point for further studies elucidating their possible role in Barrett's carcinogenesis. Copyright \u00a9 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.1576","type":"journal-article","created":{"date-parts":[[2004,5,21]],"date-time":"2004-05-21T06:01:08Z","timestamp":1085119268000},"page":"780-788","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Array\u2010based comparative genomic hybridization for the detection of DNA sequence copy number changes in Barrett's adenocarcinoma"],"prefix":"10.1002","volume":"203","author":[{"given":"Bettina","family":"Albrecht","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Hausmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Horst","family":"Zitzelsberger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hubert","family":"Stein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg R\u00fcdiger","family":"Siewert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Hopt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rupert","family":"Langer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heinz","family":"H\u00f6fler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Werner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Walch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,5,21]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1166"},{"key":"e_1_2_6_3_2","first-page":"1531","article-title":"Allelic imbalance of 7q32.3\u2013q36.1 during tumorigenesis in Barrett's esophagus","volume":"62","author":"Riegman PH","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64760-8"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2264(199802)21:2<172::AID-GCC14>3.0.CO;2-T"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2264(199908)25:4<410::AID-GCC17>3.0.CO;2-J"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddg261"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63458-X"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1359"},{"key":"e_1_2_6_10_2","first-page":"2872","article-title":"Array\u2010based comparative genomic hybridization for genome\u2010wide screening of DNA copy number in bladder tumors","volume":"63","author":"Veltman JA","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1476-5586(03)80017-9"},{"key":"e_1_2_6_12_2","first-page":"6240","article-title":"Impact of DNA amplification on gene expression patterns in breast cancer","volume":"62","author":"Hyman E","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_6_13_2","first-page":"6211","article-title":"Amplicon mapping and transcriptional analysis pinpoint cyclin L as a candidate oncogene in head and neck cancer","volume":"62","author":"Redon R","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_6_14_2","first-page":"467","article-title":"Genome wide detection of oncogene amplifications in nasopharyngeal carcinoma by array based comparative genomic hybridization","volume":"20","author":"Hui AB","year":"2002","journal-title":"Int J Oncol"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780280"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780394"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780289"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(98)01432-2"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1053\/S0046-8177(03)00354-X"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1309\/MXXH-25N3-UAL2-G7XX"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1201330"},{"key":"e_1_2_6_22_2","first-page":"1867","article-title":"Allelic loss of 9p21 and mutation of the CDKN2\/p16 gene develop as early lesions during neoplastic progression in Barrett's esophagus","volume":"13","author":"Barrett MT","year":"1996","journal-title":"Oncogene"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.1576","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.1576","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:50:50Z","timestamp":1761058250000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.1576"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,5,21]]},"references-count":21,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,7]]}},"alternative-id":["10.1002\/path.1576"],"URL":"https:\/\/doi.org\/10.1002\/path.1576","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"value":"0022-3417","type":"print"},{"value":"1096-9896","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,5,21]]},"assertion":[{"value":"2003-11-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-03-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-05-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}