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In order to do so, HNK\u20101 expression was evaluated immunohistochemically in 100 primary cutaneous melanomas and correlated with metastasis after up to 10\u2010years' follow\u2010up. Furthermore, HNK\u20101 expression was analysed in metastatic deposits (19 distant cutaneous metastases and six sentinel lymph node metastases), as well as in benign nevi. Kaplan\u2013Meier analysis revealed a positive association between HNK\u20101 expression and metastasis (<jats:italic>p<\/jats:italic> &lt; 0.005) and multivariate Cox regression analysis adjusted for the standard prognostic markers ulceration and vertical tumour thickness confirmed HNK\u20101 expression as an independent prognostic marker. HNK\u20101 expression was preserved in 42% of the distant cutaneous metastases, but metastatic cells in lymph nodes were devoid of HNK\u20101 immunoreactivity. None of the benign pigmented lesions exhibited HNK\u20101 immunoreactivity. Expression of the HNK\u20101 glycotope in cutaneous malignant melanoma is an independent prognostic marker of metastasis. Differential HNK\u20101 expression at the metastatic sites implies that its expression is modulated by the surrounding environment. As HNK\u20101 is also transiently expressed during migration of melanocyte precursor cells derived from the neural crest, recapitulation of this transient expression might occur during metastatic spread of cutaneous malignant melanoma. Copyright \u00a9 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.1595","type":"journal-article","created":{"date-parts":[[2004,7,8]],"date-time":"2004-07-08T14:05:31Z","timestamp":1089295531000},"page":"933-939","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["The developmentally regulated neural crest\u2010associated glycotope HNK\u20101 predicts metastasis in cutaneous malignant 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