{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T12:24:59Z","timestamp":1773923099769,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T00:00:00Z","timestamp":1106870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2005,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Follicular lymphoma (FL), except grade 3B, is characterized by the chromosomal translocation t(14;18)(q32;q21), which results in over\u2010expression of the Bcl\u20102 protein. Ten per\u2010cent of all FLs, however, do not show Bcl\u20102 protein expression with standard immunohistochemistry using a monoclonal Bcl\u20102 antibody against residues 41\u201354 of the Bcl\u20102 protein. In this study, the biological background of 18 Bcl\u20102\u2010negative FL cases grade I, II, or IIIa was investigated by immunohistochemical staining and western blot analysis with alternative antibodies. Bcl\u20102 protein was demonstrated in five of the 18 cases and all of these carried the t(14;18) translocation. Of the 13 cases that were Bcl\u20102 negative with alternative antibodies, 12 lacked the t(14;18) translocation. PCR and subsequent sequence analysis of cDNA demonstrated that three cases with a t(14;18) contained somatic mutations in the translocated <jats:italic>BCL2<\/jats:italic> gene, resulting in amino acid replacements in the region of the epitope recognized by the antibody. In conclusion, the majority of Bcl\u20102\u2010negative FL lack a t(14;18) but a significant subset of these tumours are false negative due to mutations in the <jats:italic>BCL2<\/jats:italic> gene. These findings may have consequences for the use of Bcl\u20102 immunohistochemistry for diagnostic purposes. Copyright \u00a9 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.1689","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T10:17:37Z","timestamp":1106907457000},"page":"329-335","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":85,"title":["Lack of Bcl\u20102 expression in follicular lymphoma may be caused by mutations in the <i>BCL2<\/i> gene or by absence of the t(14;18) translocation"],"prefix":"10.1002","volume":"205","author":[{"given":"Margit","family":"Schraders","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daphne","family":"de 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