{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:53:49Z","timestamp":1786978429765,"version":"build-2736575974"},"reference-count":13,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T00:00:00Z","timestamp":1118793600000},"content-version":"vor","delay-in-days":8719,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1981,8]]},"abstract":"Abstract<\/jats:title>\n \n A systematic ultrastructural study has been performed of the mononuclear phagocytes in granulomas induced by different types of mycobacteria, e.g. live BCG (Pasteur), irradiated\n M. leprae<\/jats:italic>\n and irradiated BCG (Pasteur) in guinea pig lymph nodes. Live BCG (Pasteur) induces a granuloma which peaks at 2\u20103 weeks and by light microscopy, a large number of the infiltrating cells have the appearance of epithelioid cells. Ultrastructurally a large proportion of these cells have a distinct appearance. They are characterised by the presence of very large nuclei, and prominent nucleoli, paucity of cytoplasmic organelles and swollen rough endoplasmic reticulum. Cells from 2 week granulomas induced by live\n M. kansasii<\/jats:italic>\n and BCG (Glaxo), had a similar ultrastructural appearance. BCG (Pasteur) granulomas slowly resolved and by light and electron microscopy fibrosis and collagen deposition was seen by 7 weeks and was very extensive in some nodes at 10 weeks.\n <\/jats:p>\n \n The nodes from M. leprae injected animals showed peak infiltration at 5 weeks and by light microscopy the infiltrating cell population was more mixed, with most of the cells having a macrophage appearance. Electron\u2010microscopy showed these infiltrating cells to be mainly activated macrophages, containing phagocytosed organisms. Nodes from irradiated BCG (Pasteur) injected animals had peak infiltration at 1 week. The light and electron microscopic appearance showed areas consisting mainly of fibroblasts and mononuclear phagocytes. Thus, the granulomas induced by\n M. leprae<\/jats:italic>\n are very different from those induced by BCG in the guinea pig. It is suggested that lymph nodes draining areas of local injection of BCG and irradiated\n M. leprae<\/jats:italic>\n could respectively form a good model in the guinea pig for cellular and biochemical studies of the granulomas of tuberculoid and lepromatous leprosy.\n <\/jats:p>","DOI":"10.1002\/path.1711340402","type":"journal-article","created":{"date-parts":[[2005,6,21]],"date-time":"2005-06-21T14:20:43Z","timestamp":1119363643000},"page":"253-265","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Experimental mycobacterial granulomas in guinea pig lymph nodes: Ultrastructural observations"],"prefix":"10.1002","volume":"134","author":[{"given":"R. B.","family":"Narayanan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Badenoch \u2010Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. L.","family":"Turk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,6,15]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"17","article-title":"The structure of mononuclear phagocytes differentiating in vitro","volume":"76","author":"Adams D. O.","year":"1974","journal-title":"Am. J. Pathol."},{"key":"e_1_2_1_3_1","first-page":"183","article-title":"Granulomatous inflammation","volume":"24","author":"Boros D.","year":"1978","journal-title":"Prog. Allergy"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711290405"},{"key":"e_1_2_1_5_1","unstructured":"Drury R. A. andWallington E. A.1967.Carleton's histological technique 4th ed. New York\/Toronto p.167."},{"key":"e_1_2_1_6_1","first-page":"36","article-title":"Granulomatous hypersensitivity","volume":"11","author":"Epstein W. L.","year":"1967","journal-title":"Prog. 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