{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:51:37Z","timestamp":1761058297264,"version":"build-2065373602"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T00:00:00Z","timestamp":1118793600000},"content-version":"vor","delay-in-days":7531,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1984,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Imposil iron\u2014dextran is an inert tracer that has been used to study mesangial uptake and clearance of macromolecular material from the glomerular circulation. Such a tracer may be a useful marker of altered mesangial function in animals with some forms of glomerulonephritis. We have studied mesangial handling of intravenously injected Imposil (50 mg\/100 g body weight) in normal rats by light, immunofluorescence and electron microscopy for up to 3 months. Mesangial cell uptake was maximal at 48\u201354 h. Extrusion and drainage of tracer to the vascular pole and distal tubule was evident at 3 days but iron was still present in mesangial cells at 3 months. Possible functional renal impairment resulting from persistent mesangially sequestered tracer was examined by measuring daily urine protein and iron excretion. A possible relationship between failure of mesangial cells to eliminate inert tracer and increasing glomerular permeability is demonstrated, suggesting that Imposil and similar inert macromolecules cannot be used for long\u2010term studies of mesangial function.<\/jats:p>","DOI":"10.1002\/path.1711440305","type":"journal-article","created":{"date-parts":[[2005,6,21]],"date-time":"2005-06-21T14:22:21Z","timestamp":1119363741000},"page":"179-187","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Persistence of inert macromolecules (imposil) in the rat mesangium and glomerular functional disturbance"],"prefix":"10.1002","volume":"144","author":[{"given":"N. P.","family":"Goode","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M.","family":"Davison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Gowland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. R.","family":"Aparicio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Shires","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,15]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1980.18"},{"key":"e_1_2_1_3_2","first-page":"75","article-title":"Localization of colloidal substances in vascular endothelium: A mechanism of tissue damage","volume":"35","author":"Benacerraf B","year":"1959","journal-title":"Am J Pathol"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1976.49"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(60)80033-0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.13.1.55"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.113.1.47"},{"key":"e_1_2_1_8_2","first-page":"18","article-title":"Comparison of the handling of ferritin and ferritin\u2010protein conjugates by the glomerular mesangium","volume":"40","author":"Takamiya H","year":"1979","journal-title":"Lab Invest"},{"key":"e_1_2_1_9_2","first-page":"526","article-title":"Uptake and transport of Imposil by the glomerular mesangium in the mouse","volume":"37","author":"Leiper JM","year":"1977","journal-title":"Lab Invest"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02892584"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.120.6.1129"},{"key":"e_1_2_1_12_2","first-page":"14","article-title":"Glomerular localization and transport of aggregated protein in mice","volume":"17","author":"Michael AF","year":"1967","journal-title":"Lab Invest"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI106901"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107577"},{"key":"e_1_2_1_15_2","first-page":"44","article-title":"Immunoelectron microscopy of the glomerular mesangial uptake and transport of aggregated human albumin in the mouse","volume":"42","author":"Lee S","year":"1980","journal-title":"Lab Invest"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.114.4.1153"},{"key":"e_1_2_1_17_2","first-page":"283","article-title":"The disappearance kinetics of soluble immune complexes prepared with reduced and alkylated antibodies and with intact antibodies in mice","volume":"35","author":"Haakenstad AO","year":"1976","journal-title":"Lab Invest"},{"key":"e_1_2_1_18_2","first-page":"293","article-title":"The glomerular deposition of soluble immune complexes prepared with reduced and alkylated antibodies and with intact antibodies in mice","volume":"35","author":"Haakenstad AO","year":"1976","journal-title":"Lab Invest"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.36.9.1081"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/25.7.1317"},{"key":"e_1_2_1_21_2","unstructured":"Thompson Park Payne. Personal communication1983."},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.134.3.7"},{"key":"e_1_2_1_23_2","first-page":"716","article-title":"Measurement of the fractional uptake of macromolecules by the renal vascular bed compared to other vascular beds","volume":"88","author":"Herbert LA","year":"1976","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_24_2","first-page":"362","article-title":"The iron\u2010loaded cell\u2014the cytopathology of iron storage. A review","volume":"91","author":"Richter GW","year":"1978","journal-title":"Am J Pathol"},{"key":"e_1_2_1_25_2","first-page":"435","article-title":"Extravascular protein in the kidney: an ultrastructural study of its relation to renal peritubular capillary permeability using protein tracers","volume":"27","author":"Venkatachalam MA","year":"1972","journal-title":"Lab Invest"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711100306"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1159\/000399235"},{"key":"e_1_2_1_28_2","first-page":"247","article-title":"Uptake of polyvinyl alcohol by macrophages in the glomerular mesangium of rats. Histologic and functional studies","volume":"111","author":"Sterzel RB","year":"1983","journal-title":"Am J Pathol"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.1711440305","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.1711440305","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T14:34:36Z","timestamp":1760970876000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.1711440305"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,11]]},"references-count":27,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1984,11]]}},"alternative-id":["10.1002\/path.1711440305"],"URL":"https:\/\/doi.org\/10.1002\/path.1711440305","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"type":"print","value":"0022-3417"},{"type":"electronic","value":"1096-9896"}],"subject":[],"published":{"date-parts":[[1984,11]]},"assertion":[{"value":"1983-11-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1984-05-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-06-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}