{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:48:31Z","timestamp":1761094111070,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T00:00:00Z","timestamp":1118793600000},"content-version":"vor","delay-in-days":6832,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1986,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An organ culture system for explants of human gastric mucosa and gastric cancer from surgical resection specimens is described. Pyloric mucosa has been successfully maintained <jats:italic>in vitro<\/jats:italic> for up to 120 h, and gastric carcinoma for up to 48 h. Morphological preservation by light and electron microscopy was good, although some simplification of the glandular pattern occurred in normal mucosa with longer periods of <jats:italic>in vitro<\/jats:italic> culture. [<jats:sup>3<\/jats:sup>H]\u2010thymidine and [<jats:sup>3<\/jats:sup>H]\u2010leucine uptake was assessed by autoradiography, and patterns of uptake resembled those described <jats:italic>in vivo<\/jats:italic> in normal gastric mucosa and gastric intestinal metaplasia. Labelling indices with [<jats:sup>3<\/jats:sup>H]\u2010thymidine were however low compared with <jats:italic>in vivo<\/jats:italic> estimates. Successful organ culture of human gastric tissue offers a useful method for investigating factors affecting growth control in normal and neoplastic gastric epithelium.<\/jats:p>","DOI":"10.1002\/path.1711500207","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T10:43:10Z","timestamp":1119436990000},"page":"127-134","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Organ culture of human gastric mucosa and gastric cancers. 1. 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