{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T04:10:07Z","timestamp":1768968607547,"version":"3.49.0"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,6,16]],"date-time":"2005-06-16T00:00:00Z","timestamp":1118880000000},"content-version":"vor","delay-in-days":6710,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1987,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Detection of leptospiral antigen using an immunoperoxidase staining (IP) procedure was carried out on fifteen samples of human liver, (nine from autopsies and six from biopsies) and nine samples of human kidneys (eight autopsies and one biopsy). The IP staining procedure to detect leptospiral antigen (<jats:italic>L. interrogans<\/jats:italic> serovar <jats:italic>icterohaemorrhagiae<\/jats:italic>) in human liver and kidney proved to be a reproducible method useful on paraffin embedded tissues after formalin, Bouin's or Helly's fluid fixation. Furthermore, the IP procedure on paraffin\u2010embedded tissue appears to have potential as an aid to the diagnosis. IP stained leptospiral antigen was detected in portal spaces of the liver, engulfed by cells of the mononuclear phagocyte system, in the interstitium of the kidney, and lining vessel walls of both liver and kidney. The results suggest that in acute human leptospirosis (<jats:italic>L. interrogans<\/jats:italic> serovar <jats:italic>icterohaemorrhagiae<\/jats:italic>) the main factors in the pathogenesis of the lesions are related to the presence of organisms and\/or their virulence, including products released by lysis.<\/jats:p>","DOI":"10.1002\/path.1711510205","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T10:47:40Z","timestamp":1119437260000},"page":"125-131","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Detection of leptospiral antigen in the human liver and kidney using an immunoperoxidase staining procedure"],"prefix":"10.1002","volume":"151","author":[{"given":"V. A. Ferreira","family":"Alves","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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H.","family":"Yasuda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"De Brito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0232-1513(86)80004-4"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1983.tb02849.x"},{"key":"e_1_2_1_4_2","first-page":"4","volume-title":"Handbook of Experimental Immunology","author":"Weir DM","year":"1967"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.3109\/10408367509107050"},{"key":"e_1_2_1_6_2","first-page":"188","article-title":"\u00dceber die pathologische Anatomie des ansteckenden Gelbsucht (Weil'sche Krankheit)","volume":"53","author":"Beitzke H","year":"1916","journal-title":"Berl Klin Wchr"},{"key":"e_1_2_1_7_2","first-page":"481","article-title":"Zur pathologischen Anatomie des infekti\u00f6sen Icterus","volume":"54","author":"Pick L","year":"1917","journal-title":"Klin Wschr"},{"key":"e_1_2_1_8_2","unstructured":"KoppischE BondWM.The morbid anatomy of human leptospirosis; a report of thirteen fatal cases. 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