{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T23:55:14Z","timestamp":1784073314669,"version":"3.55.0"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,6,16]],"date-time":"2005-06-16T00:00:00Z","timestamp":1118880000000},"content-version":"vor","delay-in-days":6437,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1987,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Using immunocytochemical techniques at the light and electron microscope levels, <jats:italic>Legionella pneumophila<\/jats:italic> and one of its extracellular proteases were located in the lungs of guinea pigs with experimental Legionnaires' disease (LD). <jats:italic>L. pneumophila<\/jats:italic> was immunostained by several peroxidase\u2010 and gold\u2010labelling methods for light and electron microscopy. The protease was immunolabelled in tissue fixed in Carnoy's fluid at the light microscopical level and on broth\u2010grown organisms at the ultrastructural level. It was not labelled in either formalin\u2010 or glutaraldehyde\u2010fixed tissue.<\/jats:p><jats:p>Using double\u2010labelling techniques, <jats:italic>L. pneumophila<\/jats:italic> and protease were located in the same section and were shown to be intimately associated with pulmonary lesions, providing strong evidence for the role of this protease in LD pneumonia.<\/jats:p>","DOI":"10.1002\/path.1711530310","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T13:19:17Z","timestamp":1119446357000},"page":"257-264","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Immunocytochemical demonstration of the association between <i>Legionella pneumophila<\/i>, its tissue\u2010destructive protease, and pulmonary lesions in experimental Legionnaires' disease"],"prefix":"10.1002","volume":"153","author":[{"given":"Ann","family":"Williams","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Baskerville","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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