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A three\u2010stage immunoperoxidase system with gold\u2013silver amplification of the diaminobenzidine substrate was used to visualize biotinylated nucleic acid hybrids. Dot\u2013blot quantification of this visualization system indicates that subpicogramme amounts of biotinylated DNA can be detected. Optimal detection of the virus genomes in the brain tissues required a microwave irradiation step prior to hybridization. JC virus genomes were observed in the nuclei of enlarged oligodendrocytes and of some bizarre astrocytes. No other cell types were found to harbour the genomes.\n                  <\/jats:p>","DOI":"10.1002\/path.1711570208","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T09:32:00Z","timestamp":1119432720000},"page":"135-140","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["JC virus genomes in progressive multifocal leukoencephalopathy: Detection using a sensitive non\u2010radioisotopic\n                    <i>in situ<\/i>\n                    hybridization method"],"prefix":"10.1002","volume":"157","author":[{"given":"Chong Gee","family":"Teo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su Yong","family":"Wong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip 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