{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T08:35:59Z","timestamp":1768552559194,"version":"3.49.0"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,6,16]],"date-time":"2005-06-16T00:00:00Z","timestamp":1118880000000},"content-version":"vor","delay-in-days":5920,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1989,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A double\u2010labelling technique combining\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization and immunocytochemistry is described which was used to characterize cells in the central nervous system containing JC virus DNA in formalin\u2010fixed, paraffin\u2010embedded tissues from four cases of progressive multifocal leukoencephalopathy. All four cases showed positive nuclear labelling for JC virus in both oligodendrocytes and astrocytes. The latter gave a strongly positive cytoplasmic staining reaction using antibodies to glial fibrillary acidic protein and vimentin. No nuclear labelling of neurones or endothelial cells was noted. The results confirm previous suggestions that glia are the main cells infected by JC virus in this disorder and show that the distribution of viral DNA in the brain is more extensive than suggested by routine microscopy alone.\n                    <jats:italic>In situ<\/jats:italic>\n                    hybridization for JC virus may be useful in confirming the diagnosis of progressive multifocal leukoencephalopathy in both surgical biopsies and post\u2010mortem brain tissue.\n                  <\/jats:p>","DOI":"10.1002\/path.1711570405","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T09:36:29Z","timestamp":1119432989000},"page":"291-297","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["The identification of cells containing JC papovavirus DNA in progressive multifocal leukoencephalopathy by combined\n                    <i>in situ<\/i>\n                    hybridization and immunocytochemistry"],"prefix":"10.1002","volume":"157","author":[{"given":"James W.","family":"Ironside","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fraser A.","family":"Lewis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Blythe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth A.","family":"Wakefield","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197312132892405"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(71)91777-6"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.1973.00490250019001"},{"key":"e_1_2_1_5_2","volume-title":"Polyomaviruses and Human Neurological Diseases","author":"Sever JL","year":"1983"},{"key":"e_1_2_1_6_2","series-title":"Demyelinating Diseases","first-page":"503","volume-title":"Handbook of Clinical Neurology","author":"Walker DL","year":"1985"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.148.3676.1477"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00685273"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/73.6.794"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00687977"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.36.4.499"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-42-1-49"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410180412"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-198709000-00005"},{"key":"e_1_2_1_15_2","first-page":"1148","article-title":"The detection of papovavirus nucleotide sequences in cortical neurons of a patient with progressive multifocal leukoencephalopathy (PML) by in situ hybridisation","volume":"25","author":"Nakamura S","year":"1985","journal-title":"Clin Neurol (Tokyo)"},{"key":"e_1_2_1_16_2","first-page":"359","article-title":"The detection of papovavirus nucleotide sequences in frozen brain tissue from a patient with progressive multifocal leukoencephalopathy by in situ hybridisation: comparison of in situ hybridisation using isotopic and biotinylated probes","volume":"37","author":"Nakamura S","year":"1985","journal-title":"Brain Nerve (Tokyo)"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490160124"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.40.2.163"},{"key":"e_1_2_1_19_2","first-page":"466","volume-title":"Molecular Cloning. A Laboratory Manual","author":"Maniatis T","year":"1982"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00688298"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4613-2297-9_16"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1985.62.4.0475"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198802043180507"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.1711570405","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.1711570405","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T17:20:09Z","timestamp":1761067209000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.1711570405"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,4]]},"references-count":22,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1989,4]]}},"alternative-id":["10.1002\/path.1711570405"],"URL":"https:\/\/doi.org\/10.1002\/path.1711570405","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"value":"0022-3417","type":"print"},{"value":"1096-9896","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,4]]},"assertion":[{"value":"1988-08-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1988-09-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-06-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}