{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T02:22:44Z","timestamp":1767838964087,"version":"3.49.0"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,6,16]],"date-time":"2005-06-16T00:00:00Z","timestamp":1118880000000},"content-version":"vor","delay-in-days":5920,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1989,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Silver\u2010binding nucleolar organizer regions (AgNORs) have been counted in sections of routinely processed paraffin\u2010embedded tissue blocks and have been shown to assist in the distinction between benign and malignant lesions. We have examined 214 benign and malignant breast lesions by this method. The AgNOR counts were fibroadenomas 1.87 + 0.20 (mean + SD; n = 39), papillomas 1.92 + 0.21 (n = 28), sclerosing adenosis 1.96 + 0.24 (n = 23), epitheliosis 2.21 + 0.30 (n = 38), lobular carcinoma\n                    <jats:italic>in situ<\/jats:italic>\n                    2.67 + 0.54 (n =9), intraduct carcinoma 3.75 + 1.33 (n = 37), and invasive carcinoma 4.22 + 1.18 (n = 40). However, the counts in 25\u201330 per cent of epitheliosis lesions and intraduct carcinomas overlapped in the region of 2\u20133 AgNOR dots per nuclear profile. The AgNOR counts in carcinomas were also compared with ploidy and growth phase fractions (S + G\n                    <jats:sub>2<\/jats:sub>\n                    + M%) by flow cytometry. Thirty\u2010three of the 46 cancers with counts over 3 AgNOR dots per nuclear profile contained aneuploid cells (&gt; 10 per cent of the total), whereas 8 of the 12 with counts below 3 comprised diploid cells only (P &lt;0.05). Similar trends were noted with regard to growth phase fractions which were 19.15 per cent + 12.31 and 13.98 per cent + 5.55, respectively, for the two groups (P&gt;0.10). We conclude that this method alone does not offer a reliable histological discriminant for malignancy in the breast. However, AgNOR counting may provide information on breast cancer prognosis supplementary to that obtained from DNA flow cytometric analyses.\n                  <\/jats:p>","DOI":"10.1002\/path.1711570407","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T09:36:29Z","timestamp":1119432989000},"page":"307-313","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":143,"title":["Silver\u2010binding nucleolar organizer regions (AgNORs) in benign and malignant breast lesions: Correlations with ploidy and growth phase by DNA flow cytometry"],"prefix":"10.1002","volume":"157","author":[{"given":"D. D.","family":"Giri","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. F.","family":"Nottingham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Lawry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. A. C.","family":"Dundas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. C. E.","family":"Underwood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1768-322X.1986.tb00452.x"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(61)91655-5"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(79)90512-5"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(83)90332-4"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0309-1651(79)90060-2"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(82)90035-0"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(86)90219-3"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1159\/000131585"},{"key":"e_1_2_1_10_2","first-page":"165","volume-title":"Trisomy 21","author":"de la Cruz FF","year":"1981"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(82)90059-0"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1159\/000132212"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(82)90020-6"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01676192"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711510203"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.40.8.885"},{"key":"e_1_2_1_17_2","first-page":"1413","article-title":"Editorial. NORs\u2010a new method for the pathologist","volume":"1","year":"1987","journal-title":"Lancet"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1988.tb01923.x"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1957.43"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1177\/31.11.6619538"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990060106"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.184.4143.1297"},{"key":"e_1_2_1_23_2","first-page":"299","article-title":"A simplified method of DNA distribution analysis","volume":"13","author":"Dean PN","year":"1980","journal-title":"Cell Tissue Kinet"},{"key":"e_1_2_1_24_2","first-page":"23","article-title":"Nucleolar silverstaining patterns related to cell cycle phase and cell generation of PHA\u2010stimulated human lymphocytes","volume":"41","author":"Field DH","year":"1984","journal-title":"Cytobios"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/hon.2900020406"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.1711570407","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.1711570407","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T17:20:04Z","timestamp":1761067204000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.1711570407"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,4]]},"references-count":24,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1989,4]]}},"alternative-id":["10.1002\/path.1711570407"],"URL":"https:\/\/doi.org\/10.1002\/path.1711570407","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"value":"0022-3417","type":"print"},{"value":"1096-9896","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,4]]},"assertion":[{"value":"1988-03-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1988-09-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-06-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}