{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T17:34:11Z","timestamp":1763746451004,"version":"build-2065373602"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T00:00:00Z","timestamp":1118793600000},"content-version":"vor","delay-in-days":5585,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1990,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Non\u2010lymphoid cells play a key role in the initiation and maintenance of cellular immune responses. Using <jats:italic>in<\/jats:italic>\u2010<jats:italic>situ<\/jats:italic> immunohistochemical techniques and a panel of monoclonal antibodies (mcabs) reactive with B5\u2010fixed, paraffin\u2010embedded liver biopsies, we analysed the non\u2010lymphoid cell component in inflammatory infiltrates in 20 cases of chronic hepatitis B virus (HBV) infection. In addition, lymphocyte subsets and HLA\u2010DR antigens were studied<\/jats:p><jats:p>Mcab KP1 labelled scattered Kupffer cells, which variably expressed HLA\u2010DR antigens. Their random distribution and lack or significant topographical association with lymphocytes suggest that classical Kupffer cells do not play a major role in cell\u2010mediated immune reactions. On the other hand, mcab Mac387 was unreactive with normal liver tissue but labelled HLA\u2010DR+ dendritic cells in areas of intralobular inflammation. On (immuno)electron microscopy, these Mac387 + dendritic cells were situated in the Disse space, where they formed close contacts with lymphocytes. Similar dendritic cells were situated at the edge of portal tracts in cases of chronic active, but not chronic persistent hepatitis. Immunostaining on serial frozen sections revealed their close topographical association with cytotoxic\/suppressor T\u2010cells, suggesting that Mac387 + HLA\u2010DR + dendritic cells play an immunomodulatory role in the effector arm of the cellular immune response that takes place in the periphery of portal tracts and the lobular parenchyma, and that involves activation and proliferation of cytotoxic T cells.<\/jats:p><jats:p>Finally, large Mac387 \u2013 HLA\u2010DR + dendritic cells expressing the LN2 marker were situated amidst helper\/inducer T\u2010cells in the centre of severely inflamed portal tracts. In analogy with their role in the lymph node, these cells are likely to act as accessory cells in the afferent arm of the immune response that involves antigen presentation to helper T\u2010cells which subsequently assist in the generation of plasma cells.<\/jats:p><jats:p>According to the types of dendritic cells and their associated T\u2010cell subsets, different functional domains involved in the afferent and efferent limbs of the immune response can be distinguished in liver tissue involved by chronic HBV infection.<\/jats:p>","DOI":"10.1002\/path.1711600308","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T10:01:02Z","timestamp":1119434462000},"page":"223-230","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Distribution of non\u2010lymphoid, inflammatory cells in chronic HBV infection"],"prefix":"10.1002","volume":"160","author":[{"given":"Joost J.","family":"van den Oord","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita","family":"de Vos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio","family":"Facchetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Delabie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris","family":"De Wolf\u2010Peeters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valeer J.","family":"Desmet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,15]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"489","article-title":"Does the immune response play a role in the pathogenesis of chronic liver disease?","volume":"112","author":"Mondelli MU","year":"1988","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840030303"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00915803"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(83)80082-1"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(83)90022-7"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1111\/j.1600-0676.1984.tb00903.x","article-title":"Analysis with OKT monoclonal antibodies of T\u2010lymphocyte subsets present in blood and liver of patients with chrunic active hepatitis","volume":"4","author":"Mariani E","year":"1984","journal-title":"Liver"},{"key":"e_1_2_1_8_2","first-page":"121","article-title":"Cellular and humoral immune reactions in chronic active liver disease. 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