{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:31:04Z","timestamp":1792589464383,"version":"4.5.0"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,6,16]],"date-time":"2005-06-16T00:00:00Z","timestamp":1118880000000},"content-version":"vor","delay-in-days":5494,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1990,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The recent discovery that brain PGP 9.5 is a ubiquitin carboxyl\u2010terminal hydrolase suggests that the role of this protein should be studied in relation to ubiquitinated cellular inclusions characteristic of several chronic human degenerative diseases.<\/jats:p><jats:p>Formalin\u2010fixed, paraffin\u2010processed sections known to contain ubiquitin\u2010protein conjugate immunoreactivity in cortical Lewy bodies, neurofibrillary tangles, Rosenthal fibres, Pick bodies, spinal inclusions in motor neurone disease, and Mallory's hyaline in alcoholic liver disease were immunostained to localize PGP 9.5. The majority of cortical Lewy bodies in diffuse Lewy body disease showed immunoreactivity for PGP 9.5. In Alzheimer's disease, only a minority of loosely arranged globose\u2010type neurofibrillary tangles were immunostained together with a minority of neurites surrounding senile plaques. In cerebeilar astrocytomas, the periphery of the majority of Rosenthal fibres was immunostained in addition to strong diffuse cytoplasmic immunostaining in some astrocytes lacking apparent Rosenthal fibres. In Pick's disease, there was no immunostaining of inclusions but there was intense immunostaining of swollen Pick cells. No spinal inclusions in motor neurone disease were stained; however, anterior horn neurones appear to show increased levels of PGP 9.5 compared with those from control cases. No immunostaining of hepatic Mallory's hyaline was demonstrable, which accords with suggestions that PGP 9.5 is a tissue\u2010specific ubiquitin C\u2010terminal hydrolase isoenzyme.<\/jats:p><jats:p>The differential detection of a ubiquitin C\u2010terminal hydrolase in different forms of ubiquitinated inclusion body in the nervous system may form the basis of a method for assessment of the staging of inclusion body biogenesis and give insight into the dynamics of inclusion body formation. In particular, it is possible that the proportion of inclusion\u2010bearing cells showing high levels of the hydrolase will provide a marker of the level of activity of the degenerative process.<\/jats:p>","DOI":"10.1002\/path.1711610210","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T10:02:34Z","timestamp":1119434554000},"page":"153-160","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":292,"title":["Ubiquitin carboxyl\u2010terminal hydrolase (PGP 9.5) is selectively present in ubiquitinated inclusion bodies characteristic of human neurodegenerative diseases"],"prefix":"10.1002","volume":"161","author":[{"given":"James","family":"Lowe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"McDermott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Landon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. John","family":"Mayer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith D.","family":"Wilkinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3029875"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.9.3033"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711550105"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(88)90296-0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(88)90081-X"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.52.1.67"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00687787"},{"key":"e_1_2_1_9_2","first-page":"569","article-title":"Isolation of a major protein component of Rosenthal fibers","volume":"130","author":"Goldman JE","year":"1988","journal-title":"Am J Pathol"},{"key":"e_1_2_1_10_2","first-page":"505","article-title":"Selective presence of ubiquitin in intracellular inclusions","volume":"134","author":"Manetto V","year":"1989","journal-title":"Am J Pathol"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-2049-2"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1985.tb03693.x"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1987.tb02384.x"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-2049-2_4"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-2049-2_5"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1983.tb08124.x"},{"key":"e_1_2_1_17_2","first-page":"91","article-title":"The immunolocalisation of protein gene product 9\u00b75 using rabbit polyclonal and mouse monoclonal antibodies","volume":"69","author":"Wilson POG","year":"1988","journal-title":"Br J Exp Pathol"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2530630"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00427a024"},{"key":"e_1_2_1_20_2","first-page":"193","article-title":"Intermediate filament\u2010ubiquitin diseases: implications for cell sanitisation","volume":"55","author":"Mayer RJ","year":"1989","journal-title":"Biochem Soc Symp"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2410809"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(90)80593-8"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2630047"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00447a039"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/338394a0"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(89)90103-1"},{"key":"e_1_2_1_27_2","first-page":"837","volume-title":"Aizheimer's Disease and Related Disorders","author":"Bancher C","year":"1989"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.1711610210","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.1711610210","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T16:07:55Z","timestamp":1761062875000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.1711610210"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1990,6]]}},"alternative-id":["10.1002\/path.1711610210"],"URL":"https:\/\/doi.org\/10.1002\/path.1711610210","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"value":"0022-3417","type":"print"},{"value":"1096-9896","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,6]]},"assertion":[{"value":"1989-12-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1990-02-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-06-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}