{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:50:03Z","timestamp":1761094203684,"version":"build-2065373602"},"reference-count":9,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T00:00:00Z","timestamp":1118793600000},"content-version":"vor","delay-in-days":5340,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1990,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Since biliary epithelial cells of the middle\u2010sized interlobular bile ducts are targets for lymphocyte\u2010mediated damage in patients with primary biliary cirrhosis (PBC), we have developed a method for isolating and maintaining these cells in short\u2010term tissue culture. Intrahepatic biliary epithelial cells were isolated from small segments of liver removed at the time of transplantation. Cells were separated from a collagenase digest by immunomagnetic separation using Dynabeads coupled to a monoclonal antibody (HEA125) specific for a biliary epithelial cell surface antigen. The yield was approximately 3 x 10<jats:sup>5<\/jats:sup> cells\/g of liver. The isolated cells were characterized morphologically and ultrastructurally using light and electron microscopy, and immunocytochemically using HEA125 and anti\u2010cytokeratin, anti\u2010vimentin and anti\u2010asialoglycoprotein receptor antibodies. By these criteria cells were judged to be identical to biliary epithelial cells from normal liver. The cells could be maintained in short\u2010term tissue culture for up to 4 weeks without loss of biliary epithelial cell markers. Availability of interlobular biliary epithelial cells will be of value in future investigations of the pathogenetic mechanisms of PBC.<\/jats:p>","DOI":"10.1002\/path.1711620312","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T10:09:26Z","timestamp":1119434966000},"page":"255-260","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Biliary epithelial cells from the liver of patients with primary biliary cirrhosis: Isolation, characterization, and short\u2010term culture"],"prefix":"10.1002","volume":"162","author":[{"given":"Ruth","family":"Joplin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. J.","family":"Strain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. M.","family":"Neuberger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,15]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"529","volume-title":"Clinical\u2010pathologic Relations in Primary Biliary Cirrhosis","author":"Shaffner F","year":"1982"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(88)80463-X"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02621273"},{"key":"e_1_2_1_5_2","first-page":"3549","article-title":"Indication, technique and results of liver graft volume reduction before orthotopic transplantation in children","volume":"19","author":"de Hemptinne B","year":"1987","journal-title":"Trans Proc"},{"key":"e_1_2_1_6_2","first-page":"617","article-title":"Extended preservation of liver for clinical transplantation","volume":"1","author":"Kalayoglu M","year":"1988","journal-title":"Lancet"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-197606000-00009"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.24.7802"},{"key":"e_1_2_1_9_2","first-page":"2883","article-title":"Immuno\u2010histochemical study of the surface expression of a M, 34,000 human epithelium\u2010specific glycoprotein in normal and malignant tissues","volume":"47","author":"Momburg F","year":"1987","journal-title":"Cancer Res"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02628499"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.1711620312","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.1711620312","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:01:39Z","timestamp":1761058899000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.1711620312"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,11]]},"references-count":9,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1990,11]]}},"alternative-id":["10.1002\/path.1711620312"],"URL":"https:\/\/doi.org\/10.1002\/path.1711620312","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"type":"print","value":"0022-3417"},{"type":"electronic","value":"1096-9896"}],"subject":[],"published":{"date-parts":[[1990,11]]},"assertion":[{"value":"1990-05-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1990-06-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-06-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}