{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T09:48:36Z","timestamp":1774691316407,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,6,16]],"date-time":"2005-06-16T00:00:00Z","timestamp":1118880000000},"content-version":"vor","delay-in-days":5190,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1991,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Mild hyperthermia is known to enhance apoptosis in a range of normal and neoplastic cell populations. Studies of tumours previously shown to respond to heating in this manner might be expected to provide insights not only into the mechanism of hyperthermic cell killing, but also into the apoptotic process in general. In the present study, cell death induced by 43\u00b0C heating for 30 min in two human Burkitt's lymphoma lines, BM 13674 and WW1, and in murine mastocytoma P\u2010815 \u00d7 2\u00b71 was found to be exclusively apoptotic in type, identification being based on light and electron microscopic appearances and on the presence of internucleosomal cleavage of DNA into fragments that are multiples of 180\u2013200 base pairs, which was demonstrated by agarose gel electrophoresis. The heat\u2010induced apoptosis was prevented by the presence of zinc sulphate, an inhibitor of the endonuclease considered to be responsible for the DNA cleavage, but was not suppressed by the protein synthesis inhibitor cycloheximide. The findings question the validity of the widely held view that active protein synthesis is an invariable prerequisite for the execution of apoptosis. It is suggested that an inositol triphosphate\u2010mediated increase in cytosolic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    , resulting from limited membrane damage, might be the critical event responsible for activation of apoptosis by mild hyperthermia.\n                  <\/jats:p>","DOI":"10.1002\/path.1711630410","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T10:16:36Z","timestamp":1119435396000},"page":"329-336","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":122,"title":["Apoptosis induced by mild hyperthermia in human and murine tumour cell lines: A study using electron microscopy and DNA gel electrophoresis"],"prefix":"10.1002","volume":"163","author":[{"given":"Yasuo S.","family":"Takano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian V.","family":"Harmon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John F. R.","family":"Kerr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)62312-8"},{"key":"e_1_2_1_3_2","first-page":"18","article-title":"Patterns of cell death","volume":"13","author":"Walker NI","year":"1988","journal-title":"Methods Achiev Exp Pathol"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711180406"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.2307\/3575245"},{"key":"e_1_2_1_6_2","first-page":"1121","article-title":"The morphological categorization of cell death induced by mild hyperthermia and comparison with death induced by ionizing radiation and cytotoxic drugs","volume":"3","author":"Allan DJ","year":"1986","journal-title":"Scan Electron Microsc"},{"key":"e_1_2_1_7_2","first-page":"311","article-title":"Kinetic and physical studies of cell death induced by chemotherapeutic agents or hyperthermia","volume":"19","author":"Dyson JED","year":"1986","journal-title":"Cell Tissue Kinet"},{"key":"e_1_2_1_8_2","first-page":"229","volume-title":"Perspectives on Mammalian Cell Death","author":"Allan DJ","year":"1987"},{"key":"e_1_2_1_9_2","first-page":"129","volume-title":"Hyperthermic Oncology 1988","author":"Harmon BV","year":"1989"},{"key":"e_1_2_1_10_2","unstructured":"HarmonBV CorderAM CollinsRJ et al.Cell death induced in a murine mastocytoma by 42 to 47\u00b0C heatingin vitro: evidence that the form of death changes from apoptosis to necrosis above a critical heat load.Int J Radiat Biol(in press)."},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1080\/09553009014552221"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/284555a0"},{"key":"e_1_2_1_13_2","first-page":"755","article-title":"Cell death","volume":"17","author":"Wyllie AH","year":"1987","journal-title":"Int Rev Cytol (Suppl)"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.132.1.38"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711420112"},{"key":"e_1_2_1_16_2","first-page":"93","volume-title":"Perspectives on Mammalian Cell Death","author":"Kerr JFR","year":"1987"},{"key":"e_1_2_1_17_2","volume-title":"Statistical Methods","author":"Snedecor GW","year":"1980"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3262923"},{"key":"e_1_2_1_19_2","first-page":"593","article-title":"Apoptosis: the role of the endonuclease","volume":"136","author":"Arends MJ","year":"1990","journal-title":"Am J Pathol"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-8326-0_32"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(86)90152-0"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711580207"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)77327-4"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.169.3.765"},{"key":"e_1_2_1_25_2","first-page":"1433","article-title":"Metabolic and ultrastructural aspects of the in vitro lysis of chronic lymphocytic leukemia cells by glucocorticoids","volume":"42","author":"Galili U","year":"1982","journal-title":"Cancer Res"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1080\/09553008814550431"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711160302"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(89)90029-1"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/337181a0"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.2307\/3577239"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.2307\/3577238"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.2307\/3577240"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/341197a0"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.1711630410","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.1711630410","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T17:53:21Z","timestamp":1761069201000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.1711630410"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,4]]},"references-count":32,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1991,4]]}},"alternative-id":["10.1002\/path.1711630410"],"URL":"https:\/\/doi.org\/10.1002\/path.1711630410","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"value":"0022-3417","type":"print"},{"value":"1096-9896","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,4]]},"assertion":[{"value":"1990-07-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1990-11-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-06-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}