{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:10:02Z","timestamp":1761059402998,"version":"build-2065373602"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,6,16]],"date-time":"2005-06-16T00:00:00Z","timestamp":1118880000000},"content-version":"vor","delay-in-days":5037,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1991,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous studies enumerating AgNORs in cutaneous melanocytic lesions have produced inconsistent results. It is probable that such inconsistencies arise from differences in fixation, staining technique, and counting strategies. Our group, having demonstrated an improved method of silver staining and having optimized counting, is now able to reconsider the role of AgNORs in evaluating borderline melanocytic lesions. Diagnostic problems similar to those encountered in routine practice have been examined. It is shown that only by counting intra\u2010 and extranucleolar AgNORs in combination with assessing the pattern of AgNOR dispersal is it possible to (1) discriminate dysplastic naevi from melanoma and (2) distinguish Spitz naevi and pigmented spindle cell naevi from melanoma. An analysis of AgNOR numbers alone results in considerable overlap between the groups studied. The value of assessing patterns of AgNOR dispersal within and outside clustered nucleolar structures is emphasized.<\/jats:p><jats:p>Lesions labelled as minimal deviation melanoma (by experts in dermatopathology) were also investigated. The majority of these specimens form a distinct group lying apart from control naevi and melanomas. This finding, whilst of interest, is difficult to evaluate because of poorly defined diagnostic criteria.<\/jats:p>","DOI":"10.1002\/path.1711650110","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T10:22:55Z","timestamp":1119435775000},"page":"61-67","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Re\u2010evaluating silver\u2010stained nucleolar organizer regions (AgNORs) in problematic cutaneous melanocytic lesions: A study with quantitation and pattern analysis"],"prefix":"10.1002","volume":"165","author":[{"given":"A. T.","family":"Evans","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. M.","family":"Orrell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Grant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"424","volume-title":"Molecular Biology of the Cell","author":"Alberts B","year":"1983"},{"key":"e_1_2_1_3_2","first-page":"191","article-title":"The nucleolar organizer regions","volume":"49","author":"Hernandez\u2010Verdun D","year":"1983","journal-title":"Biol Cell"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1159\/000131373"},{"key":"e_1_2_1_5_2","first-page":"1413","article-title":"NORs\u2010a new method for the pathologist","author":"Editorial","year":"1987","journal-title":"Lancet"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-74668-0_3"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.43.3.184"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711600202"},{"key":"e_1_2_1_9_2","first-page":"31","article-title":"Nucleolar organizer regions in fibrous proliferations of childhood and infantile fibrosarcoma","volume":"40","author":"Egan M","year":"1987","journal-title":"J Clin Pathol"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1988.tb02085.x"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1988.tb02080.x"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711540307"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1988.tb02079.x"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1989.tb02114.x"},{"key":"e_1_2_1_15_2","first-page":"1250","article-title":"Nucleolar organizer regions in transitional cell tumours of the bladder","volume":"113","author":"Cairns P","year":"1989","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1177\/030089168907500102"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00718634"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.42.12.1276"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004347-199001000-00005"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.40.8.885"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1988.tb02007.x"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1989.tb02148.x"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19890201)63:3<474::AID-CNCR2820630314>3.0.CO;2-3"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(89)90133-0"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1989.tb01324.x"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000372-199004000-00006"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1988.tb01932.x"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711580303"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711550206"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711590206"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711610205"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.43.12.1029"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711630309"},{"key":"e_1_2_1_34_2","first-page":"74","article-title":"Buffered phenol formaldehyde (pH 7\u00b70 and pH 5\u00b75): improved fixation in an enclosed tissue processor","volume":"46","author":"Slidders W","year":"1989","journal-title":"Med Lab Sci"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711510203"},{"key":"e_1_2_1_36_2","first-page":"121","article-title":"Effect of melanin bleaching on immunoperoxidase, with reference to ocular tissues and lesions","volume":"43","author":"Alexander RA","year":"1989","journal-title":"Med Lab Sci"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(86)80199-X"},{"issue":"1","key":"e_1_2_1_38_2","first-page":"339","article-title":"What are your criteria for the diagnosis of malignant Spitz's nevus' and \u2018minimal deviation melanoma\u2019?","volume":"6","author":"Reed RJ","year":"1984","journal-title":"Am J Dermatopathol"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-199001000-00006"},{"key":"e_1_2_1_40_2","first-page":"110","volume-title":"Pathobiology and Recognition of Malignant Melanoma","author":"Reed RJ","year":"1988"},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711560308"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.1711650110","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.1711650110","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:44:33Z","timestamp":1761057873000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.1711650110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,9]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1991,9]]}},"alternative-id":["10.1002\/path.1711650110"],"URL":"https:\/\/doi.org\/10.1002\/path.1711650110","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"type":"print","value":"0022-3417"},{"type":"electronic","value":"1096-9896"}],"subject":[],"published":{"date-parts":[[1991,9]]},"assertion":[{"value":"1990-12-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1991-04-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-06-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}