{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T12:46:42Z","timestamp":1770986802536,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T00:00:00Z","timestamp":1118793600000},"content-version":"vor","delay-in-days":4914,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1992,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\u03b1B crystallin is a lens protein which has homology with the small heat\u2010shock proteins and is also expressed in nonenticular tissues. Polyclonal antibodies have been raised to a synthetic peptide corresponding to residues 1\u201310 of \u03b1B crystallin. The antiserum detects a 20 kDa polypeptide on nitrocellulose replicas after polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate of extracts of heart muscle known to be rich in \u03b1B crystallin. Staining of normal human tissues reveals immunoreactivity of lens capsular epithelium, skeletal muscle, cardiac muscle, smooth muscle. renal tubular epithelium, Schwann cells, and glial cells, as has been described by other workers. In addition, positive staining of normal thyroid epithelium, colonic epithelium, and stratified squamous epithelium was seen.<\/jats:p><jats:p>Tissues known to contain ubiquitinated inclusion bodies were immunostained with the anti\u2010\u03b1B\u2010crystallin antiserum. Staining of cortical Lewy bodies, astrocytic Rosenthal fibres, and hepatic Mallory bodies was seen, but only a propertion of inclusions were positive. Neurones containing the ubiquitinated inclusions of Alzheimer's disease were only very rarely stained and the ubiquitinated inclusons of motor neurone disease were not detected by the antiserum. Reactive astrocytes in cerebral tissues were strongly immunostained.<\/jats:p><jats:p>The results suggest that \u03b1B crystallin is involved in the formation of ubiquitinated inclusion bodies that have issociated intermediate filaments and support previous observations on the localization of a brain\u2010specific ubiquitin carboxy\u2010terminal hydrolase which similarly divides ubiquitinated filamentous inclusions in the central nervous system into two main groups.<\/jats:p>","DOI":"10.1002\/path.1711660110","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T14:07:26Z","timestamp":1119449246000},"page":"61-68","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":156,"title":["\u03b1B crystallin expression in nonlenticular tissues and selective presence in ubiquitinated inclusion bodies in human disease"],"prefix":"10.1002","volume":"166","author":[{"given":"J.","family":"Lowe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"McDermott","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Pike","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Spendlove","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Landon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. J.","family":"Mayer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,15]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.57.070188.002403"},{"key":"e_1_2_1_3_2","first-page":"366","article-title":"Evolution of the lens crystallins: the stress connection","volume":"9","author":"DeJong WW","year":"1989","journal-title":"TIBS"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.7.2360"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.15.5738"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(89)80215-3"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.9.3.1083"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90173-6"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711550105"},{"key":"e_1_2_1_10_2","first-page":"505","article-title":"Selective presence of ubiquitin in intracellular inclusions","volume":"134","author":"Manetto V","year":"1989","journal-title":"Am J Pathol"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)92075-S"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1177\/38.1.2294148"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.19.6126"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(86)90337-X"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6308771"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711610210"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2990.1990.tb01263.x"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.1.90"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711640305"},{"key":"e_1_2_1_20_2","first-page":"193","article-title":"Intermediate filament\u2010ubiquitin diseases: implications for cell sanitisation","volume":"55","author":"Mayer RJ","year":"1989","journal-title":"Biochem Soc Symp"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.1711660110","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.1711660110","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T09:24:17Z","timestamp":1761038657000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.1711660110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,1]]},"references-count":19,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,1]]}},"alternative-id":["10.1002\/path.1711660110"],"URL":"https:\/\/doi.org\/10.1002\/path.1711660110","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"value":"0022-3417","type":"print"},{"value":"1096-9896","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,1]]},"assertion":[{"value":"1991-02-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1991-05-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-06-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}