{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T02:21:30Z","timestamp":1773454890008,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T00:00:00Z","timestamp":1118793600000},"content-version":"vor","delay-in-days":4670,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1992,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The ultrastructure and immunostaining with antibodies against actin, desmin, and vimentin were studied in the peritubular myofibroblasts of testes from normal men and men with Klinefelter' syndrome (KS). The seminiferous tubules were classified into five types (a\u2013e), related to the progressive degree of sclerosis measured as thickening of the lamina propria. In control testes, only types a and b tubules were present, whereas the testes from men with KS showed types b, c, d, and e tubules. The ultrastructural study revealed abundant microfilament bundles with electron\u2010dense bodies in the cell periphery of the myofibroblasts in a and b tubules. In c tubules, the microfilament bundles of the myofibroblasts were lacking in electron\u2010dense bodies. Myofibroblasts in tubules d and e showed scanty microfilament bundles. Immunostaining of peritubular myofibroblasts with anti\u2010actin antibodies was intense in tubule types a\u2013c and scanty in types d and e. Immunostaining of myofibroblasts with anti\u2010desmin antibodies was intense in tubule types a and b, and negative in types c\u2013e. Immunostaining with anti\u2010vimentin antibodies was weak in tubule types a\u2013c and intense in types d and e. Quantitative study revealed that with the progression of sclerosis, the number and volume per cross\u2010sectioned tubule of actin\u2010containing cells and, mainly, desmin\u2010containing cells decrease while the number and volume of vimentin\u2010containing cells increase.<\/jats:p>","DOI":"10.1002\/path.1711680111","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T12:06:02Z","timestamp":1119441962000},"page":"59-66","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["The peritubular myofibroblasts in the testes from normal men and men with Klinefelter's syndrome. A quantitative, ultrastructural, and immunohistochemical study"],"prefix":"10.1002","volume":"168","author":[{"given":"R.","family":"Martin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Santamar\u00eda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Nistal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Fraile","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Paniagua","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,15]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"129","article-title":"Electron microscopy of the Leydig cells and hormone assays in Klinefeller's syndrome","volume":"53","author":"Saba P","year":"1969","journal-title":"Endokrinologie"},{"key":"e_1_2_1_3_2","first-page":"671","article-title":"Elude en microscope electronique de la cellule de Leydig dans la maladie de Klinefelter en p\u00e9riodes pre, per et postpubertaires","volume":"32","author":"Rubin P","year":"1971","journal-title":"Ann Endocrinol"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711460405"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711640405"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod17.3.390"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.1990.tb01946.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00221083"},{"key":"e_1_2_1_9_2","first-page":"881","article-title":"Modulation of actin isoform expression in alveolar myofibroblasts (contractile inlerstitial cells) during pulmonary hypertension","volume":"136","author":"Kapancy Y","year":"1990","journal-title":"Am J Pathol"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/33.3.3882826"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(79)90418-X"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(86)90356-8"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092150103"},{"key":"e_1_2_1_14_2","first-page":"502","article-title":"Immunohistochemical analysis for desmin in normal and neoplastic ovarian stromal tissue","volume":"114","author":"Lastaria D","year":"1990","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_1_15_2","first-page":"101","volume-title":"Inflammation of the testis, epididymis, paratesticular membranes and scrotum","author":"Mikuz G","year":"1982"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.1986.tb00865.x"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.99.1.22s"},{"key":"e_1_2_1_18_2","first-page":"27","article-title":"Intermediate filament networks: organization and possible functions of a diverse group of cytoskeletal elements","volume":"9","author":"Goldman RD","year":"1986","journal-title":"J Cell Sci Suppl"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)69151-3"},{"key":"e_1_2_1_20_2","first-page":"119","article-title":"Morphogenetic restructuring and formation of basement membranes by Sertoli cell and testis peritubular cells in coculture. Inhibilion of Ihe morphogenetic cascade by cydic AMP\u2010derivalives and by blocking direct cell contact","volume":"123","author":"Tung PS","year":"1987","journal-title":"Dev Biol"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1177\/019262338901700219"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.1980.tb01693.x"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.1990.tb00970.x"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.1711680111","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.1711680111","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T10:40:25Z","timestamp":1761043225000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.1711680111"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,9]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,9]]}},"alternative-id":["10.1002\/path.1711680111"],"URL":"https:\/\/doi.org\/10.1002\/path.1711680111","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"value":"0022-3417","type":"print"},{"value":"1096-9896","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,9]]},"assertion":[{"value":"1992-02-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1992-04-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-06-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}