{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:14:36Z","timestamp":1770822876855,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T00:00:00Z","timestamp":1118793600000},"content-version":"vor","delay-in-days":4579,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1992,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The distinction between reactive mesothelium and mesothelioma in pleural biopsy specimens is notoriously difficult, and conventional immunohistochemical markers have provided no relief. The object of this study was to examine the frequency of immunohistochemically detectable p53 overcxpression in routinely processed, paraffin\u2010embedded tissue from pleural mesotheliomas and from pleura showing reactive mesothelial hyperplasia, using a polyclonal antibody to formalin\u2010resistant p53 epitopes, and to consider the diagnostic utility of this antibody in the distinction between mesothelioma and reactive mesothelium in pleural biopsy specimens. Immunostaining was enhanced by pepsin predigestion prior to the application of the primary antibody. Positivity occurred in 10\/16 epithelial mesotheliomas, 9\/19 biphasic mesotheliomas, 2\/12 sarcomatous mesotheliomas but in none of 20 reactive pleura. Immunostaining was particularly intense in some of the biopsy specimens, which may be due to the rapidity with which these small pieces of tissue were fixed. In conclusion, this study suggests that p53 immunostaining can help to distinguish epithelial or biphasic mesothelioma from reactive mesothelial hyperplasia in formalin\u2010fixed, paraffin\u2010embedded pleural biopsy specimens.<\/jats:p>","DOI":"10.1002\/path.1711680407","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T12:12:50Z","timestamp":1119442370000},"page":"377-381","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["p53 immunostaining in the distinction between benign and malignant mesothelial proliferations using formalin\u2010fixed paraffin sections"],"prefix":"10.1002","volume":"168","author":[{"given":"Frederick G.","family":"Mayall","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heather","family":"Goddard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allen R.","family":"Gibbs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,15]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.4.1.1"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711620103"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711660402"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711570304"},{"key":"e_1_2_1_6_2","first-page":"807","article-title":"p53 expression in colorectal tumors","volume":"138","author":"Purdie CA","year":"1991","journal-title":"Am J Pathol"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)90801-B"},{"key":"e_1_2_1_8_2","first-page":"839","article-title":"Detection of p53 in primary lung tumors and nonsmall cell lung carcinoma cell lines","volume":"139","author":"Caamano J","year":"1991","journal-title":"Am J Pathol"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)90586-E"},{"key":"e_1_2_1_10_2","first-page":"5410","article-title":"Genetic alterations of the p53 gene are a feature of malignant mesothebomas","volume":"51","author":"Cote RJ","year":"1991","journal-title":"Cancer Res"},{"key":"e_1_2_1_11_2","first-page":"62","volume-title":"Biological Effects of Asbestos","author":"Kannerstein M","year":"1973"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1136\/thx.40.2.91"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711430303"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/jso.2930450204"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1992.tb00915.x"},{"key":"e_1_2_1_16_2","unstructured":"ShuklaV JasaniB.The enhancement of p53 antigenicity in formalin\u2010 fixed tissue by pepsin predigestion. (In preparation.)"},{"key":"e_1_2_1_17_2","first-page":"260","article-title":"Diffuse mesothelioma and asbestos exposure in the North Western Cape Province","volume":"17","author":"Wagner JC","year":"1960","journal-title":"Br J Ind Med"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1136\/thx.45.9.649"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19840901)54:5<951::AID-CNCR2820540536>3.0.CO;2-A"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.1711680407","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.1711680407","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T12:23:14Z","timestamp":1761049394000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.1711680407"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,12]]},"references-count":18,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1992,12]]}},"alternative-id":["10.1002\/path.1711680407"],"URL":"https:\/\/doi.org\/10.1002\/path.1711680407","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"value":"0022-3417","type":"print"},{"value":"1096-9896","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,12]]},"assertion":[{"value":"1992-05-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1992-06-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-06-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}