{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T22:12:41Z","timestamp":1766441561778,"version":"build-2065373602"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T00:00:00Z","timestamp":1118793600000},"content-version":"vor","delay-in-days":4548,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The peritoneum has been shown to possess fibrinolytic activity which is thought to play a role in the prevention of intra\u2010abdominal adhesion formation. Recently inflamed peritoneal tissue has been shown to have reduced fibrinolytic activity secondary to increased levels of plasminogen activator inhibitor\u20101 (PAI\u20101). The aim of this study was to localize the production of PAI\u20101 in appendix tissue using\n                    <jats:italic>in situ<\/jats:italic>\n                    mRNA hybridization. Sections of normal and inflamed appendix were hybridized with a digoxigenin\u2010labelled cDNA probe. PAI\u20101 production was localized to both mcsothelium and serosal blood vessel endothelium in all inflamed appendix samples. Cell identities were confirmed using immunohistochemistry directed against mesothelial and endothlial cell markers. Staining was not seen on sections of normal appendix or on negative control slides of inflamed appendix (hybridization with plasmid DNA, PAI\u20101 probe following ribonuclease digestion). The identification of the cells expressing the PAI\u20101 gene in peritoneum increases our understanding of the pathophysiological changes in fibrinolytic activity which occur in inflammation and may lead to adhesion formation.\n                  <\/jats:p>","DOI":"10.1002\/path.1711690111","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T12:13:33Z","timestamp":1119442413000},"page":"67-71","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Localization of plasminogen activator inhibitor\u20101 production in inflamed appendix by\n                    <i>in situ<\/i>\n                    mRNA hybridization"],"prefix":"10.1002","volume":"169","author":[{"given":"Simon A.","family":"Whawell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiming","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth A.","family":"Fleming","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth M.","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy N","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,15]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"1","article-title":"Local host defence in the peritoneal cavity","volume":"2","author":"Hau T","year":"1989","journal-title":"Surg Immun"},{"key":"e_1_2_1_3_2","first-page":"81","article-title":"Fibrinolytic activity of mesothelial surfaces","volume":"20","author":"Porter JM","year":"1969","journal-title":"Sure Forum"},{"key":"e_1_2_1_4_2","first-page":"623","article-title":"Fibrinolytic activity in serosal and synovial membranes","volume":"88","author":"Myhre\u2010Jensen O","year":"1969","journal-title":"Arch Pathol"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5223(19)42001-1"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9610(73)90011-1"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4804(76)90075-5"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1159\/000128208"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.1800760422"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/181495b0"},{"key":"e_1_2_1_11_2","first-page":"52","article-title":"Tissue fibrinolytic activity studied by a histological method","volume":"25","author":"Kwaan HC","year":"1966","journal-title":"Fed Proc"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(84)80201-X"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.44.2.139"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)91125-T"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711670104"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1647636"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0019-2791(69)90288-2"},{"key":"e_1_2_1_18_2","first-page":"104","volume-title":"The unlabelled antibody peroxidaseantiperoxidase (PAP) technique. 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