{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:23:46Z","timestamp":1770823426705,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T00:00:00Z","timestamp":1118793600000},"content-version":"vor","delay-in-days":4489,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1993,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Detection of B\u2010cell monoclonality using the polymerase chain reaction (PCR) promises the quick and cost\u2010effective separation of monoclonal from polyclonal B\u2010cell disease. However, the efficiency of the method has yet to be fully assessed, particularly with regard to disease type and selection of PCR primers.<\/jats:p><jats:p>We have evaluated two approaches based on amplification of the immunoglobulin heavy chain gene using frame work 2 (Fr2) and framework 3 (Fr3) region primers. Frozen tissue samples from 94 cases of low\u2010grade B\u2010cell lymphoma were investigated, all of which had previously been shown to be monoclonal by Southern blot analysis. Using a Fr2 primer, we were able to show monoclonality in 85 per cent of cases; with Fr3, 80 per cent of cases; and using both techniques in separate reactions, 90 per cent of cases.<\/jats:p><jats:p>Thus, a significant false\u2010negative rate exists with either primer which can be reduced by using both. We also found a difference in the efficiency of detection in different types of lymphoma; only 87 percent of mucosa\u2010associated lymphomas and centroblastic\/centrocytic lymphomas were shown to be monoclonal, whereas all of the other lymphoma types tested were positive using one or both methods.<\/jats:p><jats:p>We conclude that PCR detection of B\u2010cell monoclonality allows rapid analysis of tissue samples, including paraffin\u2010processed material. False\u2010negative results which occur in some types of lymphoma can be reduced by the use of two or more primer combinations.<\/jats:p>","DOI":"10.1002\/path.1711690303","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T12:17:23Z","timestamp":1119442643000},"page":"291-295","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":185,"title":["Detection of monoclonality in low\u2010grade B\u2010cell lymphomas using the polymerase chain reaction is dependent on primer selection and lymphoma type"],"prefix":"10.1002","volume":"169","author":[{"given":"Tim C.","family":"Diss","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaizheng","family":"Peng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew C.","family":"Wotherspoon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter G.","family":"Isaacson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Langxing","family":"Pan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,15]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V75.11.2220.2220"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1990.tb02579.x"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.43.11.888"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.45.9.770"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V78.1.192.192"},{"key":"e_1_2_1_7_2","first-page":"726","article-title":"An improved method for detection of B\u2010lymphoid clonality by polymerase chain reaction","volume":"5","author":"Deane M","year":"1991","journal-title":"Leukemia"},{"key":"e_1_2_1_8_2","volume-title":"Sequences of Proteins of Immunological Interest","author":"Kabat EA","year":"1987"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.43.5.429"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.13.5123"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-199207000-00001"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-199205000-00004"},{"key":"e_1_2_1_13_2","first-page":"292","article-title":"Analysis of immunoglobulin heavy chain gene rearrangement using the polymerase chain reaction. Reply to letter","volume":"79","author":"Trainor KJ","year":"1992","journal-title":"Blood"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198701083160204"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90488-5"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1987.tb00508.x"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.176.4.1137"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.141.11.3994"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.1711690303","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.1711690303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:35:32Z","timestamp":1761057332000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.1711690303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,3]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1993,3]]}},"alternative-id":["10.1002\/path.1711690303"],"URL":"https:\/\/doi.org\/10.1002\/path.1711690303","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"value":"0022-3417","type":"print"},{"value":"1096-9896","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,3]]},"assertion":[{"value":"1992-11-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1992-12-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-06-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}