{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:48:35Z","timestamp":1761094115714,"version":"build-2065373602"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T00:00:00Z","timestamp":1118793600000},"content-version":"vor","delay-in-days":3971,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1994,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two monoclonal antibodies (FBI and FB21) reactive in formalin\u2010fixed, paraffin\u2010embedded tissue sections are reported in this paper. FB1 and FB21 recognize a cytoplasmic antigen and a surface antigen of B cells, respectively. FBI reacts with mantle zone (MZ) B cells, germinal centre (GC) cells, and marginal zone (MrZ) B cells, but not with T cells in lymphoid tissues. FB21 reacts with MZ B cells, GC cells in lymphoid tissues, and T cells of peripheral blood, but not with MrZ B cells in the spleen. Neither monoclonal antibody (MoAb) reacts with monocytes, granulocytes, or plasma cells. FB1 reacted with all the B\u2010cell lymphomas tested and with CD20\u2010positive Reed\u2010Sternberg cells in two of five cases of Hodgkin's disease, but not with multiple myelomas or T\u2010cell lymphomas. FB21 reacted with B\u2010cell lymphoma in 20 of 22 cases, but not with multiple myelomas, T\u2010cell lymphomas, or Reed\u2010Sternberg cells of Hodgkin's disease. Immunoprecipitation studies revealed that FB1 recognizes the same two polypeptide chains that are recognized by L26 and is a member of the CD20 antibody cluster. FB21 was thought to recognize a sialic acid\u2010dependent carbohydrate epitope and this was confirmed at the Fifth International Conference on Human Leukocyte Differentiation Antigens (Boston, 1993) FB21 did not react with splenic MrZ B cells and was different from the pan B markers reported previously [CD20 (L26), CD45RA (MB1), and CD74 (LN\u20102)]. FB21 recognizes a subset of B cells and appears to be closely related to CD75\/76 antibodies. FB1 and FB21 are useful MoAbs for the diagnosis and analysis of B\u2010cell lymphomas.<\/jats:p>","DOI":"10.1002\/path.1711730410","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T12:31:22Z","timestamp":1119443482000},"page":"347-354","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Production of two monoclonal antibodies (FB1 AND FB21) useful for the identification of human B lymphocytes in formalin\u2010fixed, paraffin\u2010embedded tissues"],"prefix":"10.1002","volume":"173","author":[{"given":"Yoshihiro","family":"Nozawa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masafumi","family":"Abe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Ohno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shirou","family":"Fukuhara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haruki","family":"Wakasa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,15]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1980.222"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19801215)46:12<2640::AID-CNCR2820461218>3.0.CO;2-S"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1990.tb05424.x"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/30.3.7037942"},{"key":"e_1_2_1_6_2","first-page":"676","article-title":"Methods in laboratory investigation. 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