{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:01:38Z","timestamp":1770822098870,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T00:00:00Z","timestamp":1118793600000},"content-version":"vor","delay-in-days":3787,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1995,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Expression of the p53 protein can be detected by immunohistochemistry in Reed\u2010Sternberg (RS) cells, the presumed neoplastic component of Hodgkin's disease (HD) lesions. At present, there is no clear molecular evidence that p53 positive immunostaining in HD correlates with the presence of mutations or other structural alterations of the p53 gene. To address this question, 49 cases of HD have been investigated for p53 expression by immuno\u2010histochemistry, using the DO1 monoclonal antibody on paraffin sections. Thirty\u2010seven out of 49 cases (75 per cent) exhibited positive immunostainining restricted to RS cells and variants. Among these 37 positive cases, ten cases were selected on the basis of a rich content of RS cells showing virtually 100 per cent DO1 positivity. A PCR\u2010mismatch strategy was chosen for the detection of p53 mutations. The threshold level of sensitivity was assessed on positive cell\u2010line controls. A proportion of 10\u201315 per cent p53 mutated cells mixed in a normal population could be identified. Total genomic DNA was extracted from the ten selected HD cases and PCR amplification of exons 5\u20138 of the p53 gene was performed. Heteroduplex mismatch analysis revealed no structural alterations of the p53 gene in any case. In view of these findings, it appears unlikely that the sensitivity of the method by itself can fully explain the negative results, although this possibility cannot be completely ruled out. Thus, it is conceivable that p53 positive immunostaining in HD may not necessarily imply genomic alterations in the classic \u2018hot spot\u2019 regions and may be related to another mechanism of p53 protein stabilization.<\/jats:p>","DOI":"10.1002\/path.1711750206","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T13:12:34Z","timestamp":1119445954000},"page":"189-194","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["PCR\u2010mismatch analysis of p53 gene mutation in Hodgkin's 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