{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T07:02:02Z","timestamp":1789628522686,"version":"build-2803163510"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T00:00:00Z","timestamp":1118793600000},"content-version":"vor","delay-in-days":3759,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1995,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two hundred and eight cases of B\u2010cell non\u2010Hodgkin's lymphoma (B\u2010NHL) occurring in Europeans without any signs of HIV infection were investigated for their association with an Epstein\u2010Barr virus (EBV) infection. The polymerase chain reaction (PCR) was applied for EBV\u2010DNA detection, <jats:italic>in situ<\/jats:italic> hybridization (ISH) for the cellular localization of EBV\u2010encoded small nuclear RNAs (EBER) and immediate\u2010early RNAs (BHLF), and immunohistology (IH) for the detection of EBV\u2010encoded latent membrane protein (LMP) and EBV nuclear antigen 2 (EBNA2) expression. PCR and EBER\u2010ISH produced congruent results in those cases with amplifiable DNA. EBV was present overall in 26 per cent (54\/208) of the B\u2010NHL cases. Through EBER\u2010ISH, the virus could be localized merely in rare non\u2010neoplastic bystander lymphocytes in 27 and additionally in tumour cells of 27 cases. Unexpectedly, the proportion of EBV\u2010infected tumour cells present in the different cases varied between 1 and 100 per cent. All but three of the cases with infected tumour cells were of high\u2010grade malignancy. Correlation with the morphological and immunological tumour phenotype revealed that all cases with more than 80 per cent EBER\u2010positive tumour cells were either B\u2010anaplastic large cell lymphomas (B\u2010ALCL), sporadic Burkitt's lymphomas, or B\u2010NHLs with partial or full plasmacellular differentiation. LMP was consistently absent from Burkitt's lymphomas and constantly expressed in B\u2010ALCLs with EBER\u2010positive tumour cells, while in all other instances it varied greatly and was rarer than EBER expression. The above findings suggest that in cases where EBV is present in 80\u2013100 per cent of tumour cells, EBV infection takes place before malignant transformation and thus may have contributed to the malignant phenotype, whereas in the other cases with only a smaller number of infected tumour and single bystander cells, EBV infection may have occurred following malignant transformation. In these cases, infection would appear to be of little or no significance in tumourigenesis.<\/jats:p>","DOI":"10.1002\/path.1711750303","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T13:12:36Z","timestamp":1119445956000},"page":"263-271","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":101,"title":["Epstein\u2010Barr virus in B\u2010cell non\u2010Hodgkin's lymphomas: Unexpected infection patterns and different infection incidence in low\u2010 and high\u2010grade types"],"prefix":"10.1002","volume":"175","author":[{"given":"Michael","family":"Hummel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis","family":"Anagnostopoulos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Korbjuhn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"Stein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,6,15]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(64)91524-7"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.59.1.94"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(82)90923-8"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-198309000-00012"},{"key":"e_1_2_1_6_2","first-page":"86","article-title":"Epstein\u2010Barr viral DNA in tissues of Hodgkin's disease","volume":"129","author":"Weiss LM","year":"1987","journal-title":"Am J Pathol"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V74.2.810.810"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1992.tb06945.x"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)90002-M"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V80.7.1804.1804"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V82.1.217.bloodjournal821217"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910170205"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/148.6.967"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02899428"},{"key":"e_1_2_1_15_2","first-page":"1315","article-title":"A survey of Epstein\u2010Barr virus gene expression in sporadic non\u2010Hodgkin's lymphomas. Detection of Epstein\u2010Barr virus in a subset of peripheral T\u2010cell lymphomas","volume":"140","author":"Hamilton\u2010Dutoit SJ","year":"1992","journal-title":"Am J Pathol"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.23.9006"},{"key":"e_1_2_1_17_2","first-page":"1889","volume-title":"Virology","author":"Kieff E","year":"1990"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-68-6-1575"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1177\/32.2.6198355"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(88)90367-4"},{"key":"e_1_2_1_21_2","first-page":"675","volume-title":"Neoplastic Hematopathology","author":"Stein H","year":"1992"},{"key":"e_1_2_1_22_2","first-page":"13","article-title":"High incidence of Epstein\u2010Barr virus genomes in Hodgkin's disease","volume":"137","author":"Herbst H","year":"1990","journal-title":"Am J Pathol"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V80.2.484.484"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-69-5-1051"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.145.4.1014"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6336310"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.1711750303","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.1711750303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T09:52:42Z","timestamp":1761040362000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.1711750303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,3]]},"references-count":25,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1995,3]]}},"alternative-id":["10.1002\/path.1711750303"],"URL":"https:\/\/doi.org\/10.1002\/path.1711750303","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"value":"0022-3417","type":"print"},{"value":"1096-9896","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,3]]},"assertion":[{"value":"1994-03-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1994-10-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-06-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}