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In this study we have used transmission electron microscopy (TEM) to document the precise subscellular localization of PDC\u2010E2 in BEC. Two antibodies which recognize PDC\u2010E2 were used: affinity\u2010purified anti\u2010PDC\u2010E2 raised in rabbits; and human antibody from the serum of patients with PBC, affinity\u2010purified against human heart PDC. The intracellular localization of antibody binding was determined by laser scanning confocal microscopy and TEM. Both antibodies bound to the inner membrane of mitochondria in BEC isolated from both patients with PBC and controls, but binding to the external aspect of the plasma membrane was observed only in BEC from patients with PBC. Surface antigen expression in PBC may make BEC immunological targets.<\/jats:p>","DOI":"10.1002\/path.1711760409","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T13:17:45Z","timestamp":1119446265000},"page":"381-390","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Subcellular localization of pyruvate dehydrogenase dihydrolipoamide acetyltransferase in human intrahepatic biliary epithelial cells"],"prefix":"10.1002","volume":"176","author":[{"given":"Ruth","family":"Joplin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorraine L.","family":"Wallace","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerald D.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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