{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T10:37:08Z","timestamp":1792147028428,"version":"4.4.0"},"reference-count":13,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T00:00:00Z","timestamp":1118793600000},"content-version":"vor","delay-in-days":3575,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1995,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hairy cell leukaemia (HCL) is a chronic lymphoproliferative disease of B\u2010cell lineage. One of the peculiar immunophenotypic markers is the strong expression of the p55 chain of the interleukin\u20102 receptor (IL2R), recognized by anti\u2010CD25 (or anti\u2010Tac) monoclonal antibody. However, it is known that in rare cases CD25 may not be detectable, even when variant forms of HCL are excluded. The possibility has not been investigated that in these situations CD25 is present in the cytoplasm of the neoplastic cells. This paper describes a case in which the clinical, histological, and electron microscopic features were consistent with a typical HCL. Immunophenotype analysis showed the whole spectrum of markers of HCL, except for the expression of IL2R. The soluble form of the molecule was, however, increased in the patient's serum. Cytospin staining of the neoplastic B cells with anti\u2010CD25 clearly demonstrated the presence of IL2R in the cytoplasm of hairy cells. When the cells were cultivated <jats:italic>in vitro<\/jats:italic> in the presence of interferon\u2010\u03b12b, CD25 was detectable at the membrane level. These findings suggest that at least some cases of CD25\u2010negative HCL may express cytoplasmic IL2R.<\/jats:p>","DOI":"10.1002\/path.1711770108","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T13:18:23Z","timestamp":1119446303000},"page":"41-47","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["CD25\u2010negative hairy cell leukaemia: Intracytoplasmic detection of Tac antigen and interferon\u2010induced surface expression"],"prefix":"10.1002","volume":"177","author":[{"given":"Tiziana","family":"Tison","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Dazzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Giacon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrizio","family":"Vianello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria 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