{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T12:00:49Z","timestamp":1778846449029,"version":"3.51.4"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T00:00:00Z","timestamp":1118793600000},"content-version":"vor","delay-in-days":3575,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1995,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The growth of newly formed vessels, or neoangiogenesis, represents an important step in both physiological and pathological situations: in particular, tumour growth and metastasis require angiogenesis. Microvessel count (MC), which represents a measure of tumour angiogenesis, has been associated with metastatic spread in cutaneous, mammary, prostatic, head and neck, and early\u2010stage lung cancer. In this study, the role of tumour angiogenesis as a prognostic indicator was examined in 253 primary non\u2010small cell lung cancer (NSCLC) patients. Microvessels were counted by highlighting endothelial cells with anti\u2010Factor VIII monoclonal antibody (MAb) in methacarn\u2010fixed tumour samples. In univariat analysis, MC (<jats:italic>P<\/jats:italic>&lt;0\u00b7000001), sex (<jats:italic>P<\/jats:italic>=0\u00b70036), histotype (<jats:italic>P<\/jats:italic>&lt;0\u00b7014), tumour status (<jats:italic>P<\/jats:italic>&lt;0\u00b7007), and vessel invasion (<jats:italic>P<\/jats:italic>&lt;0\u00b7019) were significantly related to hilar and\/or mediastinal nodal involvement. However, in the stepwise logistic regression analysis, MC (<jats:italic>P<\/jats:italic>&lt;0\u00b7000003) retained the most important influence on nodal metastasis. The overall survival analysis calculated by the Kaplan\u2010Meier method revealed that tumours with high MC (&gt;25 vessels\/field) were significantly associated with increased death risk (log\u2010rank test <jats:italic>P<\/jats:italic>=0\u00b700067; Cox's test <jats:italic>P<\/jats:italic>=0\u00b700046; Gehan's Wilcoxon test <jats:italic>P<\/jats:italic>=0\u00b700108). In 94 patients, the development of metastatic disease during follow\u2010up was significantly related to MC. Indeed, patients who developed metastasis during follow\u2010up showed a higher MC, either as a dichotomous (<jats:italic>P<\/jats:italic>=0\u00b701) or as a continuous (<jats:italic>P<\/jats:italic>=0\u00b7003) variable, than patients who had developed no metastasis at the time of the analysis. Moreover, in the stepwise logistic regression analysis, MC retained the most important influence on distant metastases. Microvessel count, as a method for the quantitation of tumour angiogenesis, has an important prognostic role in non\u2010small cell lung carcinomas.<\/jats:p>","DOI":"10.1002\/path.1711770110","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T13:18:23Z","timestamp":1119446303000},"page":"57-63","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":133,"title":["Microvessel count predicts metastatic disease and survival in non\u2010small cell lung cancer"],"prefix":"10.1002","volume":"177","author":[{"given":"Gabriella","family":"Fontanini","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Bigini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvana","family":"Vignati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fulvio","family":"Basolo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfredo","family":"Mussi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Lucchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvana","family":"Chine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlo 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