{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T12:19:33Z","timestamp":1786450773489,"version":"build-2736575974"},"reference-count":55,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,5,12]],"date-time":"2005-05-12T00:00:00Z","timestamp":1115856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The transcription factor PU.1 has been shown to be crucial for the early stages of B cell development but its function at later stages of B cell development is less well known. We observed previously that PU.1 is expressed uniformly throughout the mature pre\u2010plasma cell B cell population, the only exception being a subpopulation of germinal centre (GC) cells which showed exceptionally high expression of PU.1. This suggested that PU.1 may also have a role in GC B cell biology. To test this hypothesis and to screen for possible genes regulated by PU.1, we first evaluated semi\u2010quantitatively the possible co\u2010expression of PU.1 with proteins known to be upregulated or downregulated during GC B cell development. Normal lymphoid tissues and 255 B cell non\u2010Hodgkin lymphomas of putative GC B cell origin were evaluated. PU.1 expression was positively associated with CD10 (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.0001), CD20 (\n                    <jats:italic>p<\/jats:italic>\n                    = 0.043), CD22 (\n                    <jats:italic>p<\/jats:italic>\n                    = 0.005), CD79a (\n                    <jats:italic>p<\/jats:italic>\n                    = 0.024) and Bcl\u20106 (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.0001) and negatively associated with cytoplasmic immunoglobulin light\u2010chain expression (\n                    <jats:italic>p<\/jats:italic>\n                    = 0.036) in diffuse large B cell lymphoma. Identical or nearly identical associations were found in follicular lymphoma. Since\n                    <jats:italic>CD20<\/jats:italic>\n                    is known to be partly regulated by PU.1 and putative PU.1\u2010binding sites have been described in the regulatory regions of the\n                    <jats:italic>CD22, CD79a<\/jats:italic>\n                    and\n                    <jats:italic>CD10<\/jats:italic>\n                    genes, we looked for putative PU.1 binding sites in the\n                    <jats:italic>BCL6<\/jats:italic>\n                    promotor. Four such putative PU.1 binding sites were identified. Further analysis by gel\u2010shift electromobility essay showed that PU.1 protein binds to three of the four putative binding sites in the\n                    <jats:italic>BCL6<\/jats:italic>\n                    promotor. PU.1 and Bcl\u20106 were also found to be upregulated in centroblasts in the normal GC, but jointly downregulated in a subpopulation of centrocytes. Our findings support the contention that PU.1 may also have an important role in GC B cell development. Copyright \u00a9 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/path.1777","type":"journal-article","created":{"date-parts":[[2005,5,12]],"date-time":"2005-05-12T09:46:31Z","timestamp":1115891191000},"page":"312-319","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["PU.1 protein expression has a positive linear association with protein expression of germinal centre B cell genes including\n                    <i>BCL\u20106, CD10, CD20<\/i>\n                    and\n                    <i>CD22<\/i>\n                    : identification of PU.1 putative binding sites in the\n                    <i>BCL\u20106<\/i>\n                    promotor"],"prefix":"10.1002","volume":"206","author":[{"given":"Emina","family":"Torlakovic","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Malecka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"June H","family":"Myklebust","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Tierens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans\u2010Christian","family":"Aasheim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jahn 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