{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:17:47Z","timestamp":1791980267341,"version":"4.4.0"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,3,31]],"date-time":"2006-03-31T00:00:00Z","timestamp":1143763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In aspirin\u2010intolerant subjects, adverse bronchial and nasal reactions to cyclooxygenase (COX) inhibitors are associated with over\u2010production of cysteinyl\u2010leukotrienes (cys\u2010LTs) generated by the 5\u2010lipoxygenase (5\u2010LO) pathway. In the bronchi of patients with aspirin\u2010intolerant asthma, we previously linked cys\u2010LT over\u2010production and aspirin hyper\u2010reactivity with elevated immunoexpression in eosinophils of the terminal enzyme for cys\u2010LT production, LTC<jats:sub>4<\/jats:sub> synthase. We investigated whether this anomaly also occurs in the nasal airways of these patients. Immunohistochemical expression of 5\u2010LO and COX pathway proteins was quantified in nasal polyps from 12 patients with aspirin\u2010intolerant asthma and 13 with aspirin\u2010tolerant asthma. In the mucosa of polyps from aspirin\u2010intolerant asthmatic patients, cells immunopositive for LTC<jats:sub>4<\/jats:sub> synthase were four\u2010fold more numerous than in aspirin\u2010tolerant asthmatic patients (<jats:italic>p<\/jats:italic> = 0.04). There were also three\u2010fold more cells expressing 5\u2010LO (<jats:italic>p<\/jats:italic> = 0.037), with no differences in 5\u2010LO activating protein (FLAP), COX\u20101 or COX\u20102. LTC<jats:sub>4<\/jats:sub> synthase\u2010positive cell counts correlated exclusively with mucosal eosinophils (<jats:italic>r<\/jats:italic> = 0.94, <jats:italic>p<\/jats:italic> &lt; 0.001, <jats:italic>n<\/jats:italic> = 25). Co\u2010localisation confirmed that five\u2010fold higher eosinophil counts (<jats:italic>p<\/jats:italic> = 0.007) accounted for the increased LTC<jats:sub>4<\/jats:sub> synthase expression in polyps from aspirin\u2010intolerant asthmatic patients, with no alterations in mast cells or macrophages. Within the epithelium, increased counts of eosinophils (<jats:italic>p<\/jats:italic> = 0.006), macrophages (<jats:italic>p<\/jats:italic> = 0.097), and mast cells (<jats:italic>p<\/jats:italic> = 0.034) in aspirin\u2010intolerant asthmatic polyps were associated only with 2.5\u2010fold increased 5\u2010LO\u2010positive cells (<jats:italic>p<\/jats:italic> &lt; 0.05), while the other enzymes were not different. Our results indicate that a marked over\u2010representation of LTC<jats:sub>4<\/jats:sub> synthase in mucosal eosinophils is closely linked to aspirin intolerance in the nasal airway, as in the bronchial airways. Copyright \u00a9 2006 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.1979","type":"journal-article","created":{"date-parts":[[2006,3,31]],"date-time":"2006-03-31T10:16:28Z","timestamp":1143800188000},"page":"392-399","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":81,"title":["Expression of 5\u2010lipoxygenase and cyclooxygenase pathway enzymes in nasal polyps of patients with aspirin\u2010intolerant 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