{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T08:41:04Z","timestamp":1790066464629,"version":"4.0.1"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,9,18]],"date-time":"2006-09-18T00:00:00Z","timestamp":1158537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Passive Heymann nephritis (PHN) induced with heterologous antisera has been described according to various criteria, which may or may not include induction of chronic disease and proteinuria. Characteristics of the glomerular immune deposits determined by the antigenic specificities of the antisera presumably account for differences in disease outcome. In this study, the clinical and immunohistological features in the model produced with monospecific antisera were compared against megalin or receptor associated protein (RAP), two proteins that have been implicated as target antigens in PHN. Rats injected with either anti\u2010megalin or anti\u2010RAP antiserum developed typical glomerular immune deposits of PHN when examined after 7 days. Although the deposits stained for complement, none of the animals had abnormal proteinuria in this time frame. Over a longer time course (7\u201316 weeks), immune deposits persisted and proteinuria increased to pathological levels in all animals injected with anti\u2010megalin serum. By contrast, immune deposits had cleared from the kidneys of rats injected with anti\u2010RAP antiserum when examined at 7\u20138 weeks post\u2010injection and the proteinuria levels observed up to 13 weeks remained in the normal range. Additional doses of anti\u2010RAP antiserum given 4 and 17 days after the first injection did not prolong the duration of glomerular immune deposits. These results demonstrate a clear divergence in pathogenic potential of antisera generated against the two renal antigens, which suggest differences in the immune deposits linked to a soluble antigen that is non\u2010covalently bound to the podocyte membrane versus those linked to an integral membrane antigen. These observations could provide clues to the nature of the unknown glomerular autoantigen of idiopathic membranous glomerulonephritis in humans. Copyright \u00a9 2006 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.2058","type":"journal-article","created":{"date-parts":[[2006,9,18]],"date-time":"2006-09-18T07:54:36Z","timestamp":1158566076000},"page":"282-287","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Differential capacity of anti\u2010RAP and anti\u2010megalin antibodies to produce progressive passive Heymann nephritis \u2014 implications for the pathogenesis of idiopathic human membranous glomerulonephritis"],"prefix":"10.1002","volume":"210","author":[{"given":"SP","family":"Makker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Tramontano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,9,18]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V6135"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V6161"},{"key":"e_1_2_6_4_2","first-page":"1613","article-title":"Induction of Heymann nephritis with a gp330\/megalin fusion protein","volume":"148","author":"Raychowdhury R","year":"1996","journal-title":"Am J Pathol"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.5.1811"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1995.56"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)53080-5"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V15792"},{"key":"e_1_2_6_9_2","first-page":"1481","article-title":"Transcription and translation of gp600 and receptor\u2010associated protein (RAP) in active Heymann nephritis","volume":"146","author":"Makker S","year":"1995","journal-title":"Am J Pathol"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.99.6.1199"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.151.11.6500"},{"key":"e_1_2_6_12_2","first-page":"807","article-title":"Antibodies to glycolipids activate complement and promote proteinuria in passive Heymann nephritis","volume":"144","author":"Susani M","year":"1994","journal-title":"Am J Pathol"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.166.1.109"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.7.1353"},{"key":"e_1_2_6_15_2","first-page":"319","article-title":"A monoclonal antibody to brush border and passive Heymann nephritis","volume":"55","author":"Ronco P","year":"1984","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_6_16_2","first-page":"502","article-title":"Experimental glomerulonephritis in the rat induced by antibodies directed against tubular antigens. 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