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Following infection, it is assumed that minute amounts of disease\u2010associated prion protein (PrP<jats:sup>Sc<\/jats:sup>) replicate by conversion of the host cellular prion protein (PrP<jats:sup>C<\/jats:sup>). Therefore, methods of rapidly reproducing this conversion process <jats:italic>in vitro<\/jats:italic> would be valuable tools in the development of such tests. We show that one such technique, protein misfolding cyclic amplification (PMCA), can amplify vCJD PrP<jats:sup>Sc<\/jats:sup> from human brain tissue, and that the degree of amplification is dependent upon the substrate <jats:italic>PRNP<\/jats:italic> codon 129 polymorphism. Both human platelets and transgenic mouse brain are shown to be suitable alternative substrate sources, and amplified PrP<jats:sup>Sc<\/jats:sup> can be detected using a conformation\u2010dependent immunoassay (CDI), allowing the detection of putative proteinase K sensitive forms of PrP<jats:sup>Sc<\/jats:sup>. Copyright \u00a9 2007 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.2204","type":"journal-article","created":{"date-parts":[[2007,7,5]],"date-time":"2007-07-05T09:16:33Z","timestamp":1183626993000},"page":"21-26","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":81,"title":["<i>In vitro<\/i> amplification and detection of variant Creutzfeldt\u2013Jakob disease PrP<sup><i>Sc<\/i><\/sup>"],"prefix":"10.1002","volume":"213","author":[{"given":"M","family":"Jones","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"AH","family":"Peden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CV","family":"Prowse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Gr\u00f6ner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JC","family":"Manson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ML","family":"Turner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JW","family":"Ironside","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"IR","family":"MacGregor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MW","family":"Head","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,8,9]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)91412-9"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/39057"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)15486-X"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16811-6"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)69835-8"},{"key":"e_1_2_6_7_2","unstructured":"HPA Press Statement2007; 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