{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:20:45Z","timestamp":1791354045599,"version":"4.3.1"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,9,11]],"date-time":"2008-09-11T00:00:00Z","timestamp":1221091200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glomerular epithelial cell (podocyte) injury is characterized by foot process retraction, slit diaphragm reorganization, and degradation of podocyte\u2010specific proteins. However, the mechanisms underlying podocyte injury are largely unknown. The ubiquitin C\u2010terminal hydrolase\u2010L1 (UCH\u2010L1) is a key modulator of ubiquitin modification in neurons. Like neurons, UCH\u2010L1 expression was associated with an undifferentiated status in cultured human podocytes, whereas differentiation and arborization decreased UCH\u2010L1 and monoUb expression. Inhibition of UCH\u2010L1 induced time and concentration\u2010dependent process formation with \u03b1\u2010actinin\u20104 distribution to the cell membrane and processes. An immunohistochemical approach was used to evaluate whether UCH\u2010L1 expression was associated with podocyte injury in 15 different human glomerular diseases. Whereas normal kidneys expressed no UCH\u2010L1 and little ubiquitin, a subset of human glomerulopathies associated with podocyte foot process effacement (membranous nephropathy, SLE class V, FSGS) <jats:italic>de novo<\/jats:italic> expressed UCH\u2010L1 in podocyte cell bodies, nuclei, and processes. Interestingly, UCH\u2010L1 expression correlated with podocyte ubiquitin content and internalization of the podocyte\u2010specific proteins nephrin and \u03b1\u2010actinin\u20104. In contrast, minimal change glomerulonephritis, a reversible disease, demonstrated minimal UCH\u2010L1 and ubiquitin expression with intact \u03b1\u2010actinin\u20104 but internalized nephrin. Glomerular kidney diseases typically not associated with foot process effacement (SLE class IV, ANCA+ necrotizing GN, amyloidosis, IgA nephritis) expressed intermediate to no UCH\u2010L1 and ubiquitin. These studies show a role for UCH\u2010L1 and ubiquitin modification in podocyte differentiation and injury. Copyright \u00a9 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.2446","type":"journal-article","created":{"date-parts":[[2008,9,11]],"date-time":"2008-09-11T10:10:25Z","timestamp":1221127825000},"page":"452-464","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":74,"title":["A new role for the neuronal ubiquitin C\u2010terminal hydrolase\u2010L1 (UCH\u2010L1) in podocyte process formation and podocyte injury in human glomerulopathies"],"prefix":"10.1002","volume":"217","author":[{"given":"C","family":"Meyer\u2010Schwesinger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TN","family":"Meyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"M\u00fcnster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Klug","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Saleem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U","family":"Helmchen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RAK","family":"Stahl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,11,4]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000075553.33781.9F"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10157-003-0259-6"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.1.1"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1447-073x.2004.00066.x"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02716"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/12647"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)01012-7"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.06.046"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.08.011"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M314124200"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2003.08.010"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2004.05205.x"},{"key":"e_1_2_7_14_2","first-page":"5690","article-title":"Serial analysis of gene expression in non\u2010small cell lung cancer","volume":"58","author":"Hibi K","year":"1998","journal-title":"Cancer Res"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00244.2002"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(2000)77:34 <40::AID-JCB9>3.0.CO;2-6"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.11.14.9409543"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2530630"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddg211"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi972274d"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1006\/scdb.2000.0164"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V11122381"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfi124"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1097\/00041552-200207000-00012"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V133630"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5002450"},{"key":"e_1_2_7_27_2","first-page":"36","article-title":"Image processing with ImageJ","volume":"11","author":"Abramoff MMP","year":"2004","journal-title":"Biophotonics Int"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80057-X"},{"key":"e_1_2_7_29_2","first-page":"423","article-title":"Morphogenetic activity of extracellular matrices on cultured podocytes. 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