{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T14:26:55Z","timestamp":1789914415530,"version":"4.0.1"},"reference-count":55,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,12,23]],"date-time":"2008-12-23T00:00:00Z","timestamp":1229990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["30570859"],"award-info":[{"award-number":["30570859"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Shanghai Leading Academic Discipline","award":["B110"],"award-info":[{"award-number":["B110"]}]}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ubiquitin C\u2010terminal hydrolase\u2010L1 (UCH\u2010L1), an important member of de\u2010ubiquitination enzyme families involved in the ubiquitin\u2013proteasome pathway, is expressed mainly in neural and reproductive systems as well as in some tumours. Recently, expression of UCH\u2010L1 has been discovered in parietal epithelial cells of Bowman's capsules and some tubular epithelia in the kidney. However, whether UCH\u2010L1 is expressed in the capillary tufts of the glomeruli has not yet become clear. In this study, we used immunohistochemistry, double immunofluorescence labelling, immunoelectron microscopy in kidney biopsy tissues and western blot in cultured rat podocytes to investigate the expression of UCH\u2010L1 and the regulation of this expression in podocytes. The results demonstrated that UCH\u2010L1 was expressed in podocytes and its expression was significantly higher in acute proliferative glomerulonephritis (APGN), lupus nephritis (LN), membranous glomerulonephritis (MGN) and IgA nephropathy than that in focal segmental glomerulosclerosis (FSGS), minimal change disease (MCD), minor abnormality and normal kidney tissues (<jats:italic>p<\/jats:italic> &lt; 0.05). In <jats:italic>in vitro<\/jats:italic> experiments, western blot showed that UCH\u2010L1 expression significantly increased in the two groups of podocytes co\u2010cultured with mesangial cells exposed to ATS 50 \u00b5l\/ml and ATS 50 \u00b5l\/ml with normal human serum 30 \u00b5l\/ml, respectively (<jats:italic>p<\/jats:italic> &lt; 0.05), while the other groups treated with TGF\u03b21 1 ng\/ml, TNF\u03b1 10 ng\/ml or IL\u20101 10 ng\/ml had little rise of UCH\u2010L1 expression, with no statistical significance compared with normal control (<jats:italic>p<\/jats:italic> &gt; 0.05). Further tests indicated that the percentage of PCNA\u2010positive podocytes in LN, APGN and IgA nephropathy was significantly higher than that in MCD, FSGS and normal control (<jats:italic>p<\/jats:italic> &lt; 0.05). These data show for the first time that podocytes do express UCH\u2010L1 and that its expression can be increased in these immunocomplex\u2010mediated nephrites. The immune injury is a main cause for stimulating podocytes to express UCH\u2010L1. The expression of UCH\u2010L1 may be associated with the regeneration of podocytes as a repair response to immunocomplex\u2010mediated injury. Copyright \u00a9 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.2511","type":"journal-article","created":{"date-parts":[[2008,12,23]],"date-time":"2008-12-23T05:34:20Z","timestamp":1230010460000},"page":"642-653","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["UCH\u2010L1 expression of podocytes in diseased glomeruli and <i>in vitro<\/i>"],"prefix":"10.1002","volume":"217","author":[{"given":"Yuan","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajing","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijuan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianzhan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hualei","family":"Gan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruixi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhonghua","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muyi","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhigang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,2,12]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2530630"},{"key":"e_1_2_6_3_2","first-page":"233","article-title":"Proteolysis and the cell cycle","volume":"1","author":"Duncan JC","year":"2002","journal-title":"Cell Cycle"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/80384"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/12647"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1530\/REP-07-0079"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V11122381"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711610210"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M314124200"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.06.046"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65169-3"},{"key":"e_1_2_6_12_2","first-page":"192","article-title":"PGP 9.5 as a marker for invasive colorectal cancer","volume":"8","author":"Yamazaki T","year":"2002","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-0824"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2005.06.014"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2006.00202.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfi124"},{"key":"e_1_2_6_17_2","volume-title":"Organogenesis of the Kidney","author":"Saxen L","year":"1997"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1080\/01913120701829285"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(99)00117-2"},{"key":"e_1_2_6_20_2","first-page":"207","article-title":"Culture of rat mesangial cell","volume":"22","author":"Zhang M","year":"1995","journal-title":"Acta Acad Med Shanghai"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellbi.2004.06.003"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2004.00209.x"},{"key":"e_1_2_6_23_2","first-page":"241","article-title":"Preparation of anti\u2010Thy1 serum and establishment of mesangioproliferative glomerulonephritis model in rat","volume":"12","author":"Cheng GP","year":"1996","journal-title":"J Clin Exp Pathol"},{"key":"e_1_2_6_24_2","first-page":"230","article-title":"Effects of sublytic complement C5b\u20109 complex on the proliferation of glomerular mensangial cell associated with NF\u2010\u03baB activation","volume":"26","author":"Tong JX","year":"2006","journal-title":"Acta Univ Med Nanjing (Nat Sci)"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00240371"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5000410"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.106.5.649"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2005.00574.x"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200112367"},{"key":"e_1_2_6_30_2","first-page":"8","article-title":"Diagnostic and prognostic significance of glomerular epithelial cell vacuolization and podocyte effacement in children with minimal lesion nephrotic syndrome and focal segmental glomerulosclerosis: an ultrastructural study","volume":"30","author":"Chiang ML","year":"1988","journal-title":"Clin Nephrol"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00146.2004"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V7122518"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2613.1999.00098.x"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000120559.09189.82"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2990.2001.00313.x"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1987.240"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1159\/000183857"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-065X.2001.1800104.x"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0711515105"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110529"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V122261"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2003.08.010"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.037077"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V10151"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5001577"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000102468.37809.C6"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2005.00161.x"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200421004"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2005020159"},{"key":"e_1_2_6_50_2","first-page":"127","article-title":"The significance of urinary podocytes in patients with active lupus nephritis","volume":"46","author":"Li JZ","year":"2007","journal-title":"Zhonghua Nei Ke Za Zhi"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2003.00723.x"},{"key":"e_1_2_6_52_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2003.00877.x"},{"key":"e_1_2_6_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5001577"},{"key":"e_1_2_6_54_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2000.00213.x"},{"key":"e_1_2_6_55_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2001.00057.x"},{"key":"e_1_2_6_56_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205390"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.2511","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.2511","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.2511","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:29:23Z","timestamp":1761060563000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.2511"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,2,12]]},"references-count":55,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2009,4]]}},"alternative-id":["10.1002\/path.2511"],"URL":"https:\/\/doi.org\/10.1002\/path.2511","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"value":"0022-3417","type":"print"},{"value":"1096-9896","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,2,12]]},"assertion":[{"value":"2008-06-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-12-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-02-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}