{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T20:06:29Z","timestamp":1761163589225,"version":"build-2065373602"},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Gene array studies indicated that\n                    <jats:italic>osteopontin<\/jats:italic>\n                    (OPN) mRNA is highly expressed in adrenocortical carcinomas (ACCs). OPN enhances invasiveness, proliferation, and metastasis formation, and is associated with poor survival in some malignant diseases. Integrin \u03b1v\u03b23 has been shown to mediate OPN effects on invasion. In this study, we demonstrated OPN and integrin \u03b1v\u03b23 expression in normal adrenal glands and benign adenomas, with staining seen exclusively in adrenocortical cells as well as even stronger staining in ACC. Western blot analysis confirmed overexpression of OPN in ACC (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.01). With Matrigel invasion assays, we have shown that OPN greatly stimulates the invasiveness of NCI\u2010h295 cells (&gt;six\u2010fold increase,\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001). Transfection with integrin \u03b1v\u03b23 further increased invasiveness after OPN stimulation (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001). This increase was reversed by the addition of an anti\u2010integrin \u03b23 antibody, indicating a functional relationship of OPN and integrin \u03b1v\u03b23 in ACC. With tissue arrays, we confirmed high OPN expression in 147 ACC samples. However, no association with survival was seen in Kaplan\u2010Meier analysis including 111 patients with primary tumours graded for OPN staining and follow\u2010up data available. In conclusion, our\n                    <jats:italic>in vitro<\/jats:italic>\n                    data indicate that OPN and integrin \u03b1v\u03b23 may act as a functional complex facilitating the invasiveness of adrenocortical tumours. This relationship remains of relevance to our understanding of carcinogenesis, but further studies are needed to address the physiological and pathological function of OPN in adrenal tissue. Copyright \u00a9 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/path.2528","type":"journal-article","created":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T17:58:19Z","timestamp":1233251899000},"page":"232-240","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Osteopontin stimulates invasion of NCI\u2010h295 cells but is not associated with survival in adrenocortical carcinoma"],"prefix":"10.1002","volume":"218","author":[{"given":"Dirk","family":"Weismann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliane","family":"Briese","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joscha","family":"Niemann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Gr\u00fcneberger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Adam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie","family":"Hahner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Johanssen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shereen","family":"Ezzat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Saeger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana\u2010Maria","family":"Bamberger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Fassnacht","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heinrich M","family":"Schulte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvia L","family":"Asa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Allolio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph M","family":"Bamberger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,3,26]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2265.2003.01881.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1677\/erc.1.01130"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2006-0706"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2005-2639"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00268-001-0030-7"},{"key":"e_1_2_6_7_2","unstructured":"National Cancer Institute.Third national cancer survey: incidental data. 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