{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T11:01:31Z","timestamp":1789642891563,"version":"4.0.0"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2010,10,4]],"date-time":"2010-10-04T00:00:00Z","timestamp":1286150400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>There have been 173 cases of variant Creutzfeldt\u2013Jakob disease (vCJD) in the UK, as of 5 July 2010, as a result of the bovine spongiform encephalopathy epidemic. The number of individuals subclinically infected with vCJD, and thus the eventual number of cases, remains, however, uncertain. In an attempt to address this problem, 63 007 tonsil tissue specimens were previously tested by enzyme immunoassay (EIA) for the presence of disease\u2010related prion protein (PrP<jats:sup>res<\/jats:sup>) and found to be negative. To confirm the reliability of this result, all those in the birth cohort most at risk (1961\u20131985) and a few others, including controls, have now been tested by immunohistochemistry (IHC). Histological slides were prepared from 10 075 anonymized formalin\u2010fixed, paraffin\u2010embedded tissues and examined for PrP<jats:sup>res<\/jats:sup> with two anti\u2010prion protein antibodies, ICMS35 and KG9. One specimen showed a single strongly positive follicle with both antibodies, on two slides from adjacent sections. As this specimen was negative when it was further investigated by EIA, IHC, and immunoblotting, it is unclear whether the patient from whom the tonsil came will go on to develop vCJD. If, however, this is the case, then a finding of 1 out of 9160 gives a prevalence of disease\u2010related prion protein in the British population of 109 per million, with a 95% confidence interval (CI) of 3\u2013608 per million, which is not statistically different (exact <jats:italic>p<\/jats:italic> = 0.63) from population prevalence estimates based on finding three positives out of 10 278 in a previous IHC study of appendix tissue. If this is not the case, a finding of 0 out of 9160 gives a prevalence of 0\u2013403 per million (95% CI) for the 1961\u20101985 cohort, which is also not different (exact <jats:italic>p<\/jats:italic> = 0.25) from previous population prevalence estimates. Therefore, the results of this work could be summarized as finding, by IHC, no or one vCJD\u2010positive individual. Copyright \u00a9 2010 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.2767","type":"journal-article","created":{"date-parts":[[2010,10,4]],"date-time":"2010-10-04T03:51:59Z","timestamp":1286164319000},"page":"380-387","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Large\u2010scale immunohistochemical examination for lymphoreticular prion protein in tonsil specimens collected in Britain"],"prefix":"10.1002","volume":"222","author":[{"given":"Mar Fernandez","family":"de Marco","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Linehan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O Noel","family":"Gill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan P","family":"Clewley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Brandner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,10,4]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/39057"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/38925"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.20708"},{"key":"e_1_2_8_5_2","unstructured":"The National Creutzfeldt\u2013Jakob Disease Surveillance Unit [Internet]; [Accessed July 5 2010]. 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