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Previously we reported that glycans on HEV\u2010like vessels in the latter lesion served as <jats:sc>L<\/jats:sc>\u2010selectin ligands, although their function is unclear. We have investigated sialyl Lewis X (sLeX)\u2010related glycoepitopes and found that MECA\u201079<jats:sup>\u2212<\/jats:sup>\/HECA\u2010452<jats:sup>+<\/jats:sup>\/NCC\u2010ST\u2010439<jats:sup>+<\/jats:sup> HEV\u2010like vessels preferentially mark gastric MALT lymphoma compared to chronic <jats:italic>H. pylori<\/jats:italic> gastritis. We then constructed CHO cell lines expressing potential MECA\u201079<jats:sup>\u2212<\/jats:sup>\/HECA\u2010452<jats:sup>+<\/jats:sup>\/NCC\u2010ST\u2010439<jats:sup>+<\/jats:sup> glycans, as well as other sLeX\u2010type glycans, on CD34 and evaluated <jats:sc>L<\/jats:sc>\u2010selectin binding to those cells, using <jats:sc>L<\/jats:sc>\u2010selectin\u2013IgM chimera binding and lymphocyte adhesion assays. <jats:sc>L<\/jats:sc>\u2010selectin\u2013IgM chimeras bound to CHO cells expressing 6\u2010sulpho\u2010sLeX attached to core 2\u2010branched <jats:italic>O<\/jats:italic>\u2010glycans with or without 6\u2010sulpho\u2010sLeX attached to extended core 1 <jats:italic>O<\/jats:italic>\u2010glycans, but only marginally to other CHO cell lines. By contrast, CHO cells expressing 6\u2010sulpho\u2010sLeX attached to extended core 1 and\/or core 2\u2010branched <jats:italic>O<\/jats:italic>\u2010glycans, as well as non\u2010sulphated sLeX attached to core 2\u2010branched <jats:italic>O<\/jats:italic>\u2010glycans, showed substantial lymphocyte binding, while binding was negligible on lines expressing 6\u2010sulpho\u2010 and non\u2010sulphated sLeX attached to <jats:italic>N<\/jats:italic>\u2010glycans and non\u2010sulphated sLeX attached to extended core 1 <jats:italic>O<\/jats:italic>\u2010glycans. These results indicate that MECA\u201079<jats:sup>\u2212<\/jats:sup>\/HECA\u2010452<jats:sup>+<\/jats:sup>\/NCC\u2010ST\u2010439<jats:sup>+<\/jats:sup> glycans, specifically, 6\u2010sulpho\u2010 and non\u2010sulphated sLeXs attached to core 2\u2010branched <jats:italic>O<\/jats:italic>\u2010glycans, expressed on HEV\u2010like vessels in gastric MALT lymphoma function as <jats:sc>L<\/jats:sc>\u2010selectin ligands and likely contribute to <jats:italic>H. pylori<\/jats:italic>\u2010specific T cell recruitment in the progression of gastric MALT lymphoma. Copyright \u00a9 2011 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.2851","type":"journal-article","created":{"date-parts":[[2011,1,5]],"date-time":"2011-01-05T07:13:21Z","timestamp":1294211601000},"page":"67-77","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Prominent expression of sialyl Lewis X\u2010capped core 2\u2010branched <i>O<\/i>\u2010glycans on high endothelial venule\u2010like vessels in gastric MALT 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