{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:40:12Z","timestamp":1790808012845,"version":"4.1.0"},"reference-count":64,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,8,24]],"date-time":"2011-08-24T00:00:00Z","timestamp":1314144000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Intramural Research Program of the National Institutes of Health"},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Centre for Cancer Research"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Kaposi's sarcoma\u2010associated herpesvirus (KSHV) encodes a viral interleukin 6 (vIL\u20106) that mimics many activities of human IL\u20106 (hIL\u20106). Both vIL\u20106 and hIL\u20106 play important roles in stimulating the proliferation of tumours caused by KSHV. Here, we provide evidence that a miRNA pathway is involved in regulation of vIL\u20106 and hIL\u20106 expression through binding sites in their open reading frames (ORFs). We show a direct repression of vIL\u20106 by hsa\u2010miR\u20101293 and hIL\u20106 by hsa\u2010miR\u2010608. The repression of vIL\u20106 by miR\u20101293 was reversed by disruption of the vIL\u20106 miR\u20101293 seed match through the introduction of point mutations. In addition, expression of vIL\u20106 or hIL\u20106 in KSHV\u2010infected cells could be enhanced by transfection of the respective miRNA inhibitors.\n                    <jats:bold>\n                      <jats:italic>In situ<\/jats:italic>\n                    <\/jats:bold>\n                    hybridization of human lymph node sections revealed that miR\u20101293 is primarily expressed in the germinal centre but is deficient in the mantle zone of lymph nodes, where the expression of vIL\u20106 is often found in patients with KSHV\u2010associated multicentric Castleman's disease, providing evidence of an anatomical correlation. Taking these factors together, our study indicates that IL\u20106 expression can be regulated by miRNA interactions in its ORF and provides evidence for the role of these interactions in the pathogenesis of KSHV\u2010associated diseases. Copyright \u00a9 2011 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/path.2962","type":"journal-article","created":{"date-parts":[[2011,7,4]],"date-time":"2011-07-04T10:31:42Z","timestamp":1309775502000},"page":"378-389","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Kaposi's sarcoma\u2010associated herpesviral IL\u20106 and human IL\u20106 open reading frames contain miRNA binding sites and are subject to cellular miRNA regulation"],"prefix":"10.1002","volume":"225","author":[{"given":"Jeong\u2010Gu","family":"Kang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Majerciak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas 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