{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T14:52:19Z","timestamp":1788015139657,"version":"build-2784847793"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,2,17]],"date-time":"2012-02-17T00:00:00Z","timestamp":1329436800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In 20\u201340% of cervical intra\u2010epithelial neoplasia (CIN) and in 4\u20138% of cervical carcinoma tissue specimens, multiple HPV genotypes have been detected. Whole tissue section (WTS) PCR does not determine how the individual types relate causally to complex and multiple CIN. Our objective was to determine whether laser capture micro\u2010dissection (LCM) with HPV PCR genotyping (LCM\u2010PCR) could accurately recover type\u2010specific HPV DNA from epithelial cells in individual areas of CIN and normal epithelium, and whether one or more viruses are present in one lesion. For that, histologically selected samples of CIN and normal epithelium were isolated by LCM and analysed by the SPF<jats:sub>10<\/jats:sub> PCR\/LiPA<jats:sub>25<\/jats:sub> (version 1) HPV genotyping system for 25 HPV genotypes. HPV genotypes detected in 756 areas of CIN (grade 1, 2 or 3) by LCM\u2010PCR were compared with results obtained by WTS\u2010PCR in 60 cases (74 biopsies). We showed that when a single HPV type is detected by WTS\u2010PCR, that type was almost always (94%; 29\/31) recovered by LCM\u2010PCR from CIN. When multiple HPV types were present by WTS\u2010PCR, their distribution within histological sections could be mapped by LCM\u2010PCR. Association of a single HPV type with a discrete area of CIN was found for 93% (372\/399) of LCM fragments analysed by PCR. We found colliding CIN lesions associated with separate HPV types and only 62% (61\/99) of HPV types detected by WTS\u2010PCR were found in CIN by LCM\u2010PCR. Therefore, the LCM\u2010PCR technique was found very accurate for high\u2010resolution HPV genotyping and for assigning an individual HPV type to an area of CIN. At LCM level, in cervical biopsy sections with multiple HPV infections, the relation between HPV types and CIN lesions is often complex. Almost every HPV type found in CIN by LCM\u2010PCR is associated with a biological separate independent CIN lesion\u2014one virus, one lesion. Copyright \u00a9 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.3970","type":"journal-article","created":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T12:40:08Z","timestamp":1322743208000},"page":"62-71","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":181,"title":["One virus, one lesion\u2014individual components of CIN lesions contain a specific HPV type"],"prefix":"10.1002","volume":"227","author":[{"given":"Wim","family":"Quint","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Jenkins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anco","family":"Molijn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Struijk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miekel","family":"van de Sandt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Doorbar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johann","family":"Mols","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Van Hoof","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Hardt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Struyf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte","family":"Colau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,2,17]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6601024"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6600688"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.22527"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kwn036"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.57.1.68"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)61248-4"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60946-5"},{"key":"e_1_2_8_9_2","first-page":"295","article-title":"Laser capture microdissection and PCR for analysis of human papilloma virus infection","volume":"293","author":"Chew K","year":"2005","journal-title":"Methods Mol Biol"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-0260"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2009.05.006"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.8.2508-2517.1999"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00539-06"},{"key":"e_1_2_8_14_2","unstructured":"TjalmaW Human papillomavirus type distribution in cervical neoplasia: results from two parallel epidemiological studies in women with high\u2010grade cervical intraepithelial neoplasia and invasive cervical cancer in Europe. 26th International Papillomavirus Conference and Clinical Workshop Montreal Quebec Canada July 3\u20138 2010; P\u2010766."},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68439-0"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1155\/2007\/874795"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.5858\/2007-131-1343-PAKIIA"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200211000-00001"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.855"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65688-X"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1155\/2007\/613150"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(92)90232-R"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01299-07"},{"key":"e_1_2_8_24_2","first-page":"4","article-title":"It is more complicated than you think: doctor, which HPV type caused that lesion?","volume":"20","author":"Stoler MH","year":"2010","journal-title":"HPV Today"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpath.3970","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.3970","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T12:07:58Z","timestamp":1761048478000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.3970"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2,17]]},"references-count":23,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,5]]}},"alternative-id":["10.1002\/path.3970"],"URL":"https:\/\/doi.org\/10.1002\/path.3970","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"value":"0022-3417","type":"print"},{"value":"1096-9896","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2,17]]},"assertion":[{"value":"2011-08-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-11-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-02-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}