{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T07:43:00Z","timestamp":1771832580317,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pentraxin 3 (PTX3) is a soluble pattern recognition receptor that binds with high affinity and selectivity to fibroblast growth factor\u20102 (FGF2), thus inhibiting its pro\u2010angiogenic activity. Here we investigated the effects of PTX3 on monoclonal gammopathy of undetermined significance (MGUS) and multiple myeloma (MM) patient\u2010derived bone marrow (BM) plasma cells (PCs), endothelial cells (ECs), and fibroblasts (FBs), and assessed whether PTX3 can modulate the cross\u2010talk between PCs and those microenvironment cells. PTX3 and FGF2 expression was evaluated by ELISA. Functional studies, including cell viability, wound healing, chemotaxis, and Matrigel<jats:sup>\u00ae<\/jats:sup> assays, were performed on MGUS and MM ECs and FBs upon the PTX3 treatment. Through western blot PTX3\u2010induced modulation in FGF2\/FGF receptor signalling pathways was evaluated in MGUS and MM ECs and FBs through western blot. Co\u2010cultures between MM ECs\/FBs and human PC lines were used to evaluate possible PTX3 indirect effects on MM PCs. Adhesion molecules were studied by flow cytometry. PTX3 provides a direct time\u2010 and dose\u2010dependent apoptotic effect on MM ECs and FBs, but not on either MM primary PCs or human PC lines. PTX3 inhibits migration of MM ECs and FBs in a dose\u2010dependent manner, and impacts <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> FGF2\u2010mediated MM angiogenesis. Co\u2010cultures of PCs and ECs\/FBs show that PTX3 treatment indirectly impairs PC viability and adhesion. We conclude that PTX3 is an anti\u2010angiogenic factor in MM and behaves as a cytotoxic molecule on MM cells by inhibiting the cross\u2010talk between PCs and ECs\/FBs. Copyright \u00a9 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.4081","type":"journal-article","created":{"date-parts":[[2012,7,27]],"date-time":"2012-07-27T18:54:13Z","timestamp":1343415253000},"page":"87-98","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Pentraxin 3 (PTX3) inhibits plasma cell\/stromal cell cross\u2010talk in the bone marrow of multiple myeloma 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