{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:27:58Z","timestamp":1791329278531,"version":"4.3.1"},"reference-count":55,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,12,17]],"date-time":"2012-12-17T00:00:00Z","timestamp":1355702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2013,1]]},"abstract":"Abstract<\/jats:title>Mucinous ovarian carcinomas (MCs<\/jats:styled-content>) typically do not respond to current conventional therapy. We have previously demonstrated amplification of HER2<\/jats:styled-content><\/jats:italic> in 6 of 33 (18.2%) mucinous ovarian carcinomas (MCs<\/jats:styled-content>) and presented anecdotal evidence of response with HER2<\/jats:styled-content>\u2010targeted treatment in a small series of women with recurrent HER2<\/jats:styled-content><\/jats:italic>\u2010amplified (HER2<\/jats:styled-content>+) MC<\/jats:styled-content>. Here, we explore HER2<\/jats:styled-content><\/jats:italic> amplification and KRAS<\/jats:styled-content><\/jats:italic> mutation status in an independent cohort of 189 MCs<\/jats:styled-content> and 199 mucinous borderline ovarian tumours (MBOTs<\/jats:styled-content>) and their association to clinicopathological features. HER2<\/jats:styled-content> status was assessed by immunohistochemistry (IHC<\/jats:styled-content>), FISH<\/jats:styled-content>, and CISH<\/jats:styled-content>, and interpreted per ASCO<\/jats:styled-content>\/CAP<\/jats:styled-content> guidelines, with intratumoural heterogeneity assessment on full sections, where available. KRAS<\/jats:styled-content><\/jats:italic> mutation testing was performed with Sanger sequencing. Stage and grade were associated with recurrence on both univariate and multivariate analysis (p<\/jats:italic> < 0.001). Assessment of HER2<\/jats:styled-content> status revealed overexpression\/amplification of HER2<\/jats:styled-content> in 29\/154 (18.8%) MCs<\/jats:styled-content> and 11\/176 (6.2%) MBOTs<\/jats:styled-content>. There was excellent agreement between IHC<\/jats:styled-content>, FISH<\/jats:styled-content>, and CISH<\/jats:styled-content> assessment of HER2<\/jats:styled-content> status (perfect concordance of HER2<\/jats:styled-content> 0 or 1+ IHC<\/jats:styled-content> with non\u2010amplified status, and 3+ IHC<\/jats:styled-content> with amplified status). KRAS<\/jats:styled-content><\/jats:italic> mutations were seen in 31\/71 (43.6%) MCs<\/jats:styled-content> and 26\/33 (78.8%) MBOTs<\/jats:styled-content>, and were near mutually exclusive of HER2<\/jats:styled-content><\/jats:italic> amplification. In the 189 MC<\/jats:styled-content> cases, a total of 54 recurrences and 59 deaths (53 of progressive disease) were observed. Within MCs<\/jats:styled-content>, either HER2<\/jats:styled-content> amplification\/overexpression or KRAS<\/jats:styled-content><\/jats:italic> mutation was associated with decreased likelihood of disease recurrence (p<\/jats:italic> = 0.019) or death (p<\/jats:italic> = 0.0041) when compared to cases with neither feature. Intratumoural heterogeneity was noted in 26% of HER2<\/jats:styled-content>\u2010overexpressing cases. These data support the stratification of MCs<\/jats:styled-content> for the testing of new treatments, with HER2<\/jats:styled-content>\u2010targeted therapy as a viable option for HER2<\/jats:styled-content>+ advanced or recurrent disease. Further research is required to delineate the molecular and clinical features of the \u223c34% of MC<\/jats:styled-content> cases with neither HER2<\/jats:styled-content><\/jats:italic> amplification nor KRAS<\/jats:styled-content><\/jats:italic> mutations.<\/jats:p>","DOI":"10.1002\/path.4088","type":"journal-article","created":{"date-parts":[[2012,8,16]],"date-time":"2012-08-16T06:03:16Z","timestamp":1345096996000},"page":"111-120","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":180,"title":["Molecular characterization of mucinous ovarian tumours supports a stratified treatment approach with HER2<\/scp> targeting in 19% of carcinomas"],"prefix":"10.1002","volume":"229","author":[{"given":"Michael S","family":"Anglesio","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of British Columbia Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Kommoss","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of British Columbia Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary C","family":"Tolcher","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Mayo Clinic Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blaise","family":"Clarke","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Galletta","sequence":"additional","affiliation":[{"name":"Australian Ovarian Cancer Study Group Peter MacCallum Cancer Centre Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry","family":"Porter","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of British Columbia Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sambasivarao","family":"Damaraju","sequence":"additional","affiliation":[{"name":"Canadian Breast Cancer Foundation Tumor Bank, Alberta Health Services and Laboratory Medicine and Pathology University of Alberta Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sian","family":"Fereday","sequence":"additional","affiliation":[{"name":"Australian Ovarian Cancer Study Group Peter MacCallum Cancer Centre Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris J","family":"Winterhoff","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Mayo Clinic Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve E","family":"Kalloger","sequence":"additional","affiliation":[{"name":"Genetic Pathology Evaluation Centre, VGH and UBC Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janine","family":"Senz","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of British Columbia Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Winnie","family":"Yang","sequence":"additional","affiliation":[{"name":"Centre for Translational and Applied Genomic, BC Cancer Agency Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Steed","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, and Division of Gynecologic Oncology University of Alberta Edmonton Alberta 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