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We sought to validate antibodies in order to define the expression and distribution of <jats:styled-content style=\"fixed-case\">MYC<\/jats:styled-content> in mature normal lymphoid cells by multiparametric immunophenotyping. Having validated two antibodies for flow cytometry and for immunohistochemistry, we analysed normal tonsil tissue. <jats:styled-content style=\"fixed-case\">MYC<\/jats:styled-content> is expressed predominantly in B cells, some of which are interfollicular large, activated, and cycling <jats:styled-content style=\"fixed-case\">CD30<\/jats:styled-content>+, <jats:styled-content style=\"fixed-case\">IRF4<\/jats:styled-content>+, <jats:styled-content style=\"fixed-case\">AID<\/jats:styled-content>\u00b1\u2009blasts. Follicular mantle, isotype\u2010switched memory B cells and <jats:styled-content style=\"fixed-case\">FcRH4<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">IRTA1<\/jats:styled-content>+ B cells express <jats:styled-content style=\"fixed-case\">MYC<\/jats:styled-content> in a wide range of levels and are small non\u2010proliferating <jats:styled-content style=\"fixed-case\">CDKN1B<\/jats:styled-content>\/p27\u2010positive or \u2010negative resting B lymphocytes. Germinal centre founder cells, <jats:styled-content style=\"fixed-case\">CD30<\/jats:styled-content>+ <jats:styled-content style=\"fixed-case\">BCL6<\/jats:styled-content>\u00b1\u2009<jats:styled-content style=\"fixed-case\">AID<\/jats:styled-content>\u00b1\u2009germinal centre blasts, and a population of <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> cells in the apical light zone express <jats:styled-content style=\"fixed-case\">MYC<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">MYC<\/jats:styled-content> is expressed in all phases of the cell cycle in activated and mature B cells, but rarely in other lymphoid types and only partially fulfils the predictions derived from extractive and <jats:italic>ex vivo<\/jats:italic> experiments of the past 30\u2009years. Copyright \u00a9 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.<\/jats:bold><\/jats:p>","DOI":"10.1002\/path.4141","type":"journal-article","created":{"date-parts":[[2012,11,20]],"date-time":"2012-11-20T04:47:44Z","timestamp":1353386864000},"page":"430-440","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["<scp>MYC<\/scp> expression and distribution in normal mature lymphoid cells"],"prefix":"10.1002","volume":"229","author":[{"given":"Giorgio","family":"Cattoretti","sequence":"first","affiliation":[{"name":"Department of Pathology AO San Gerardo  Monza Italy"},{"name":"Department of Surgical Sciences Universit\u00e1 degli Studi di Milano Bicocca  Milano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.21.6497"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2231"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.189365.112"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.09.006"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.16.1.653"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1201197"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05953"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.03.003"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113034"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0437996100"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.3.971"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1985.tb03885.x"},{"key":"e_1_2_7_14_1","first-page":"797","article-title":"c\u2010myc protein expression in untransformed fibroblasts","volume":"6","author":"Waters CM","year":"1991","journal-title":"Oncogene"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R600017200"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1563"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI45851"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V74.5.1517.1517"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830270135"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(82)90159-3"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19881115)62:10<2085::AID-CNCR2820621003>3.0.CO;2-R"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e318244e2ba"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1986.123"},{"key":"e_1_2_7_24_1","first-page":"29","article-title":"c\u2010myc protein in normal tissue. 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